AI Article Synopsis
- The mRNA differential display technique was utilized to identify genes in Habanero pepper seedlings affected by Phytophthora capsici infection, showing that multiple transcripts accumulate rather than a single gene being newly synthesized.
- Levels of the nitrate reductase (CcNR) gene increased significantly a few hours post-inoculation and were also elevated after treatment with salicylic acid and ethephon, indicating a potential protective role in response to infection.
- The study suggests that the regulation of CcNR and its enzymatic activity is linked to host defense mechanisms, influenced by both the oomycete and certain phytohormones.
Article Abstract
The mRNA differential display technique was used to identify genes from Habanero pepper (Capsicum chinense Jacq.) seedlings whose expression is modified systemically by infection with the oomycete Phytophthora capsici L. Experiments with different oligonucleotide primer combinations revealed that no single gene was synthesised de novo. Instead, the quantitative accumulation of multiple transcripts was found. From these transcripts, levels of a nitrate reductase (Capsicum chinense nitrate reductase, CcNR), which has a high percentage of identity with other Solanaceae NRs, showed a consistent increase a few hours after inoculation (hai) with P. capsici. Reverse northern blotting revealed the existence of basal levels of CcNR transcripts in different adult tissues; however, systemic levels rose dramatically after spraying seedlings with salicylic acid (SA) and ethephon (ET) but not with methyl jasmonate (MeJa). Both P. capsici and defence phytohormones (DP) also modified NR enzymatic activity (nitrite:NAD(+) oxidoreductase; EC 1.7.1.1) with similar kinetics. Because the application of DP induced and activated the CcNR differentially, it is possible that the activity of CcNR is related to a specific host defence response.
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| http://dx.doi.org/10.1016/j.plaphy.2011.05.006 | DOI Listing |
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