AI Article Synopsis
- EBV-immortalized B lymphocyte cell lines are valuable for studying various diseases, including rare genetic disorders, and can be used to generate induced pluripotent stem cells (iPSCs).
- The study presents a nonviral method for creating iPSCs from these B-cell lines associated with inherited diseases, differing from protocols used for patient fibroblasts.
- The resulting iPSCs maintained pluripotency, preserved inherited mutations, and could differentiate into all three germ layers, paving the way for new human disease models and novel therapeutic approaches.
Article Abstract
EBV-immortalized B lymphocyte cell lines have been widely banked for studying a variety of diseases, including rare genetic disorders. These cell lines represent an important resource for disease modeling with the induced pluripotent stem cell (iPSC) technology. Here we report the generation of iPSCs from EBV-immortalized B-cell lines derived from multiple inherited disease patients via a nonviral method. The reprogramming method for the EBV cell lines involves a distinct protocol compared with that of patient fibroblasts. The B-cell line-derived iPSCs expressed pluripotency markers, retained the inherited mutation and the parental V(D)J rearrangement profile, and differentiated into all 3 germ layer cell types. There was no integration of the reprogramming-related transgenes or the EBV-associated genes in these iPSCs. The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158714 | PMC |
| http://dx.doi.org/10.1182/blood-2011-03-340620 | DOI Listing |
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