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The Use of CHROMID Colistin R for the Detection of Colistin-Resistant Gram-Negative Bacteria in Positive Blood Cultures.
Antibiotics (Basel)
March 2024
Microbiology Department, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.
The aim of this study was to assess the utility of CHROMID Colistin R for direct detection of colistin-resistant Gram-negative bacteria from positive blood cultures. A total of 390 blood cultures from hospitalised patients containing Gram-negative bacteria were included in this study. These blood cultures were referred to clinical laboratories in the United Kingdom and Türkiye.
View Article and Find Full Text PDFEvaluation of three protocols for direct susceptibility testing for gram negative-Enterobacteriaceae from patient samples in Uganda with SMS reporting.
Sci Rep
February 2024
Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda.
In Uganda, the challenge of generating and timely reporting essential antimicrobial resistance (AMR) data has led to overreliance on empirical antibiotic therapy, exacerbating the AMR crisis. To address this issue, this study aimed to adapt a one-step AMR testing protocol alongside an SMS (Short Message Service) result relay system (SRRS), with the potential to reduce the turnaround time for AMR testing and result communication from 4 days or more to 1 day in Ugandan clinical microbiology laboratories. Out of the 377 samples examined, 54 isolates were obtained.
View Article and Find Full Text PDFComparison of Five Different Selective Agar for the Detection of Vancomycin-Resistant .
Antibiotics (Basel)
March 2023
Institute for Medical Microbiology, Immunology and Hygiene, University Hospital of Cologne, 50935 Cologne, Germany.
Five commercially available selective agar were evaluated regarding sensitivity and specificity to detect vancomycin-resistant () . Altogether 187 strains were included, comprising 119 -carrying strains (phenotypically vancomycin-resistant = 105; phenotypically vancomycin-susceptible VVE-B = 14) and 68 vancomycin-susceptible isolates. Limit of detection was calculated for each selective agar for pure cultures, stool suspensions and artificial rectal swabs.
View Article and Find Full Text PDFPan-Chromosome and Comparative Analysis of Reveal Important Traits Concerning the Genetic Diversity, Evolutionary Dynamics, and Niche Adaptation of the Species.
Microbiol Spectr
February 2023
MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, People's Republic of China.
Agrobacterium fabrum has been critical for the development of plant genetic engineering and agricultural biotechnology due to its ability to transform eukaryotic cells. However, the gene composition, evolutionary dynamics, and niche adaptation of this species is still unknown. Therefore, we established a comparative genomic analysis based on a pan-chromosome data set to evaluate the genetic diversity of .
View Article and Find Full Text PDFAcquisition of Enterobacterales carrying the colistin resistance gene mcr following travel to the tropics.
J Travel Med
February 2023
Bacteriology Laboratory, Bichat-Claude Bernard Hospital, AP-HP Nord-Université Paris Cité, F-75018 Paris, France.
Background: Colistin is an antibiotic of last resort in the management of highly drug-resistant Enterobacterales infections. Travel to some destinations presents a high risk of acquiring multidrug-resistant Enterobacterales, but little data are available on the risk of acquiring colistin-resistant strains. Here, we use the VOYAG-R sample collection (2012-2013) in order to evaluate the rate of acquisition of colistin-resistant Enterobacterales, excluding species with intrinsic resistance (CRE), following travel to tropical regions.
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