[Development of the certified reference material of mercury in lyophilized human urine].

Objective: To develop the certified reference material of mercury in lyophilized human urine.

Methods: Human urine samples from normal level mercury districts were filtered, homogenized, dispensed, lyophilized and radio-sterilized. Homogeneity test, stability inspection and certification were conducted using a atom fluorescence spectrophotometric method. The physical and chemical stability of the certified reference material were assessed for 18 months. The certified values are based on analysis made by three independent laboratories.

Results: The certified values are as follows: low level was (35.6 ± 2.1) µg/L, high level was (50.5 ± 3.0) µg/L.

Conclusion: The certified reference material of mercury in lyophilized human urine in this research reached the national certified reference material requirements and could be used for the quality control.