Development of microsatellite loci for Blastophaga javana (Agaonidae), the pollinating wasp of Ficus hirta (Moraceae).

Am J Bot

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Published: February 2011

Premise Of The Study: To investigate genetic structure of the pollinator and, indirectly, gene flow in its plant host, Blastophaga javana microsatellite primers were developed.

Methods And Results: Ten polymorphic microsatellite loci were developed using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol. Numbers of alleles per locus ranged from 2 to 19, with observed and expected heterozygosities ranging from 0.000 to 0.800 and from 0.000 to 0.925, respectively.

Conclusions: These markers may be useful for further investigation of population genetics of Blastophaga javana and other congeneric species.

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http://dx.doi.org/10.3732/ajb.1000432DOI Listing

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Development of microsatellite loci for Blastophaga javana (Agaonidae), the pollinating wasp of Ficus hirta (Moraceae).

Am J Bot

February 2011

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Premise Of The Study: To investigate genetic structure of the pollinator and, indirectly, gene flow in its plant host, Blastophaga javana microsatellite primers were developed.

Methods And Results: Ten polymorphic microsatellite loci were developed using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol. Numbers of alleles per locus ranged from 2 to 19, with observed and expected heterozygosities ranging from 0.

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