Pressure perturbation calorimetry (PPC) is a new technique that makes possible to study the volumetric changes that occur upon protein unfolding. Here, we summarize the thermodynamic foundation of the method and introduce a two-state model for the analysis of the unfolding data monitored by PPC. Several examples of data analysis illustrating potential pitfalls and solutions are discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S0076-6879(09)66022-5 | DOI Listing |
Publication Analysis
Top Keywords
pressure perturbation
8
perturbation calorimetry
8
calorimetry characterize
4
characterize volumetric
4
volumetric properties
4
properties proteins
4
proteins pressure
4
calorimetry ppc
4
ppc technique
4
technique study
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!