The extended Le(a)-Le(x) oligosaccharide, expressed as a cell surface antigen by human squamous lung carcinomas marks cancer cells of tumors having poor prognosis. In order to see if the extended Le(a)-Le(x) also has a precisely controlled pattern of expression during embryogenesis, a survey of representative vertebrate embryos and fetuses in various stages was undertaken. Embryonic and fetal cells which express the epitope are derived from embryonic endoderm. No. epitope expression was demonstrated in tissues of ectodermal or mesodermal origin. Vertebrate conservation of this endodermal cell surface carbohydrate suggests function necessary for normal growth and development with inappropriate re-expression during carcinogenesis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3892/or.1.3.607 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enzymatic modular assembly of hybrid Lewis antigens.
Org Biomol Chem
September 2021
National Glycoengineering Research Center, NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao 266237, China.
The enzymatic synthesis of hybrid Lewis antigens including KH-1 (Lewis y-Lewis x-Lactose, Le-Le-Lac), Lewis a-Lewis x-Lactose (Le-Le-Lac), and Lewis b-Lewis x-Lactose (Le-Le-Lac) has been achieved using a facile enzymatic modular assembly strategy. Starting from a readily available tetrasaccharide, 3 complex hybrid Lewis antigens were achieved in over 40% total yields in less than 5 linear steps of sequential enzymatic glycosylation using 6 enzyme modules. The regio-selective fucosylation was achieved by simply controlling the donor-acceptor ratio.
View Article and Find Full Text PDFMolecular and structural basis for Lewis glycan recognition by a cancer-targeting antibody.
Biochem J
September 2020
School of Science, RMIT University, Melbourne, Victoria, Australia.
Immunotherapy has been successful in treating many tumour types. The development of additional tumour-antigen binding monoclonal antibodies (mAbs) will help expand the range of immunotherapeutic targets. Lewis histo-blood group and related glycans are overexpressed on many carcinomas, including those of the colon, lung, breast, prostate and ovary, and can therefore be selectively targeted by mAbs.
View Article and Find Full Text PDFEvidence for two populated conformations for the dimeric Le(x) and Le(a)Le(x) tumor-associated carbohydrate antigens.
J Med Chem
February 2014
Department of Chemistry, University of Guelph, Guelph, Ontario, N1G2W1, Canada.
The conformational behavior of tumor-associated carbohydrate antigens (TACAs) dimLe(x) and Le(a)Le(x) was studied using a combination of NMR experiments and molecular dynamics simulations. It is shown that within the hexasaccharides, the Le(x) and Le(a) branched trisaccharide fragments adopt the rigid "stacked" conformation known for the isolated trisaccharide antigens. In contrast, the β-D-GlcNAc-(1→3)-D-Gal glycosidic bond that connects the two Le(x) trisaccharides in dimLe(x), and the Le(a) trisaccharide to the Le(x) trisaccharide in Le(a)Le(x), was found to be very flexible in both hexasaccharides.
View Article and Find Full Text PDFPopulation-based study reveals new risk-stratification biomarker panel for Barrett's esophagus.
Gastroenterology
October 2012
Medical Research Council Cancer Cell Unit, Hutchison-MRC Research Centre, Cambridge, United Kingdom.
Background & Aims: The risk of progression of Barrett's esophagus (BE) to esophageal adenocarcinoma (EAC) is low and difficult to calculate. Accurate tools to determine risk are needed to optimize surveillance and intervention. We assessed the ability of candidate biomarkers to predict which cases of BE will progress to EAC or high-grade dysplasia and identified those that can be measured in formalin-fixed tissues.
View Article and Find Full Text PDFSynthesis of Le(a)Le(x) oligosaccharide fragments and efficient one-step deprotection.
Carbohydr Res
June 2010
Department of Chemistry, University of Guelph, Guelph, Ontario, Canada.
We describe here the synthesis of two oligosaccharide fragments of the tumor associated carbohydrate antigen Le(a)Le(x). While the linear lacto-N-triose I: beta-D-Galp-(1-->4)-beta-D-GlcNAcp-(1-->3)-beta-D-Galp-OMe is a known compound, this is the first reported preparation of the branched tetrasaccharide beta-D-GlcNAcp-(1-->3)-beta-D-Galp-(1-->4)-[alpha-l-Fucp-(1-->3)]-beta-D-GlcNAcp-OMe. Our synthetic schemes involved using an N-trichloroacetylated trichloroacetimidate glucosaminyl donor activated with excess TMSOTf at 0 degrees C for glycosylation at O-3 of galactosyl residues and that of trichloroacetimidate galactosyl donors activated with excess BF(3).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!