Objective: To explore the role of Ca(2+) in nanosecond steep pulse (NSP)-induced apoptosis of human ovarian carcinoma cell line SKOV3 in vitro.
Methods: The early apoptotic rate of SKOV3 cells treated with NSP was detected by Annexin V/PI double staining and flow cytometry. MTT assay was used to detect the viability of the cells pretreated with BAPTA-AM (0, 25, 50 and 100 µmol/L) chelation for 1 h to increase the intracellular free Ca(2+) prior to NSP exposure, and the cell morphological changes and caspase 12 expression were detected using Hoechst 33342 staining and Western blotting, respectively.
Results: Flow cytometry showed that NSP induced early apoptosis of SKOV3 cells, and the optimal effect was achieved with the treatment parameter configuration of field strength of 90 kV/cm, pulse width of 100 ns, frequency of 1 Hz, and exposure time of 30 s. The highest early apoptotic rate and necrosis rate was (60.31∓5.67)% and (1.35∓0.39)%, respectively. Pretreatment with BAPTA-AM chelation prior to NSP exposure significantly increased the cell viability (P<0.05), and resulted also in lowered apoptosis rate and decreased expression of caspase 12 (P<0.05).
Conclusion: NSP can induce apoptosis in SKOV3 cells. Increased intracellular free Ca(2+) functions as an important mediator in NSP-induced cell apoptosis, which may also involve Ca(2+)-mediated endo- plasmic reticulum pathway.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Efficient Magnetic Vortex Acceleration by femtosecond laser interaction with long living optically shaped gas targets in the near critical density plasma regime.
Sci Rep
February 2024
Institute of Plasma Physics and Lasers-IPPL, University Research and Innovation Centre, Hellenic Mediterranean University, 74100, Rethymno, Greece.
We introduce a novel, gaseous target optical shaping laser set-up, capable to generate short scale length, near-critical target profiles via generated colliding blast waves. These profiles are capable to maintain their compressed density for several nanoseconds, being therefore ideal for laser-plasma particle acceleration experiments in the near critical density plasma regime. Our proposed method overcomes the laser-target synchronization limitations and delivers energetic protons, during the temporal evolution of the optically shaped profile, in a time window of approximately 2.
View Article and Find Full Text PDFUltrathin materials for wide bandwidth laser ultrasound generation: titanium dioxide nanoparticle films with adsorbed dye.
Nanoscale Adv
August 2023
CQC-IMS, Department of Chemistry, University of Coimbra 3004-535 Coimbra Portugal
Materials that convert the energy of a laser pulse into heat can generate a photoacoustic wave through thermoelastic expansion with characteristics suitable for improved sensing, imaging, or biological membrane permeation. The present work involves the production and characterization of materials composed of an ultrathin layer of titanium dioxide (<5 μm), where a strong absorber molecule capable of very efficiently converting light into heat (5,10,15,20-tetrakis(4-sulfonylphenyl)porphyrin manganese(iii) acetate) is adsorbed. The influence of the thickness of the TiO layer and the duration of the laser pulse on the generation of photoacoustic waves was studied.
View Article and Find Full Text PDFSub-Nanosecond Switching of Si:HfO Ferroelectric Field-Effect Transistor.
Nano Lett
February 2023
Viterbi Faculty of Electrical and Computer Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
The discovery of ferroelectric doped HfO enabled the emergence of scalable and CMOS-compatible ferroelectric field-effect transistor (FeFET) technology which has the potential to meet the growing need for fast, low-power, low-cost, and high-density nonvolatile memory, and neuromorphic devices. Although HfO FeFETs have been widely studied in the past few years, their fundamental switching speed is yet to be explored. Importantly, the shortest polarization time demonstrated to date in HfO-based FeFET was ∼10 ns.
View Article and Find Full Text PDFPlanar Gas Expansion under Intensive Nanosecond Laser Evaporation into Vacuum as Applied to Time-of-Flight Analysis.
Entropy (Basel)
November 2022
Federal Research Center "Computer Science and Control" of the Russian Academy of Sciences, Vavilova Str. 44/2, Moscow 119333, Russia.
A computational investigation of the dynamics of gas expansion due to intense nanosecond laser evaporation into vacuum has been carried out. The problem is solved in a one-dimensional approximation, which simplifies calculations and at the same time allows one to analyze the main features of the expansion dynamics. For analysis we use three different approaches.
View Article and Find Full Text PDFMechanistic Understanding of DNA Denaturation in Nanoscale Thermal Gradients Created by Femtosecond Excitation of Gold Nanoparticles.
ACS Appl Mater Interfaces
January 2022
Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory Code 6900, Washington, D.C. 20375, United States.
There is significant interest in developing photothermal systems that can precisely control the structure and function of biomolecules through local temperature modulation. One specific application is the denaturation of double-stranded (ds) DNA through femtosecond (fs) laser pulse optical heating of gold nanoparticles (AuNPs); however, the mechanism of DNA melting in these systems is not fully understood. Here, we utilize 55 nm AuNPs with surface-tethered dsDNA, which are locally heated using fs laser pulses to induce DNA melting.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!