Eighteen STR loci and one sex determination locus present in the Finnzymes Canine 2.1 STR Multiplex Reagent Kit were screened in the UK dog population providing allele frequencies and population genetic parameters necessary for the application of STRs to forensic genetic casework. A total of 375 dogs were genotyped, including representative samples from each of twelve breeds used to evaluate Hardy-Weinberg equilibrium and calculate inter-population pairwise F(ST) values. Three loci were excluded from calculations of average random match probability due to deviations from Hardy-Weinberg Equilibrium or ambiguous genotyping. Random match probability based on fifteen STR loci and one sex locus was subsequently estimated to be 2.8 × 10(-17) for unrelated individuals across breeds.
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http://dx.doi.org/10.1016/j.fsigen.2011.04.015 | DOI Listing |
R Soc Open Sci
January 2025
Department of Forensic Medicine, School of Basic Medical Sciences, Central South University, No172. Tongzipo Road, Changsha, Hunan 410013, People's Republic of China.
DNA mixtures containing semen and vaginal fluid are common biological samples in forensic analysis. However, the analysis of semen-vaginal fluid mixtures remains challenging. In this study, to solve these problems, it is proposed to combine semen-specific CpG sites and closely related microhaplotype sites to form a new composite genetic marker (semen-specific methylation-microhaplotype).
View Article and Find Full Text PDFForensic Sci Int Genet
December 2024
BGI Forensic, Shenzhen 518083, China. Electronic address:
In this study, we developed and validated a novel microhaplotype (MH) panel, the FGID Microhaplotype Kit, which contains 232 loci and was specifically designed for forensic kinship analysis. The performance of the panel was evaluated through rigorous testing that included sensitivity, species specificity, inhibitor resistance, uniformity, stability, accuracy and mixture deconvolution. The results showed that the kit is capable of reliably detecting all loci with minimal DNA input.
View Article and Find Full Text PDFForensic Sci Int Genet
January 2025
Forensic DNA Division, National Forensic Service, Wonju, South Korea. Electronic address:
Y-chromosomal short tandem repeats (Y-STRs) at rapidly mutating (RM) loci have been suggested as tools for differentiating paternally related males. RMplex is a recently developed system that incorporates 26 RM loci and four fast-mutating (FM) loci, targeting 44 male-specific loci. Here, we evaluated the RMplex by estimating Y-STR mutation rates and the overall differentiation rates for 542 Korean father-son pairs, as well as the genetic population values for 409 unrelated males.
View Article and Find Full Text PDFActa Parasitol
January 2025
Veterinary Laboratories, PAAFR, P.O. Box: 21422, Safat, Kuwait, 13075, Kuwait.
Purpose: The objective of the study was to establish the prevalence of Sarcocystis (Apicomplexa, Sarcocystidae) in brown rats from Jleeb Al-Shuyoukh, Kuwait, and to describe detected parasites using morphological and DNA analysis methods.
Methods: Ninety-eight brown rats (Rattus norvegicus) were examined for Sarcocystis spp. Obtained sarcocysts were investigated using light microscopy and electron microscopy.
Genes (Basel)
December 2024
China National Rodent Laboratory Animal Resources Center, Beijing 102629, China.
The genetic quality of laboratory mice may have a direct impact on the results of research. Therefore, it is essential to improve genetic monitoring methods to guarantee research quality. However, few current methods boast high efficiency, high throughput, low cost, and general applicability at the same time.
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