Sub-10 nm nanopores drilled by a focused electron beam in a transmission electron microscope are widely used in solid-state nanopore devices for DNA translocation. However, there still remains much controversy surrounding the drilling mechanism. In order to explain the drilling of nanopores by electrons, we undertook a theoretical consideration of the energy transfer from the fast electrons to the solid through such mechanisms as elastic and inelastic scattering. According to the calculations based on the scattering cross-section, the direct atomic displacement cross-section induced by elastic scattering increases with increasing incident electron energy, while the ionization cross-section and temperature increment decrease. We performed nanopore drilling in a Si3N4 membrane using two different electron energies, 200 and 300 kV, to identify the drilling mechanism. The dependence of the nanopore drilling on the incident electron energy was well matched with the direct atomic displacement.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1088/0957-4484/22/27/275303 | DOI Listing |
Nanoscale
December 2024
Computational Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany.
Nanopores drilled in materials can electrophoretically drive charged biomolecules to enable their detection. Here, we explore and compare two-dimensional nanopores, graphene and MoS, in order to unravel their advantages and disadvantages with regard to protein detection. We tuned the protein translocation and its dynamics by the choice and concentration of the surrounding solvent.
View Article and Find Full Text PDFInt J Mol Sci
October 2024
Institute of Biomedical Chemistry, 10, Pogodinskaya St., 119121 Moscow, Russia.
ACS Omega
October 2024
State Key Laboratory of Oil & Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu, Sichuan 610500, China.
Environ Sci Technol
November 2024
Faculty of Engineering, China University of Geosciences, Wuhan, Hubei 430074, China.
Nano Lett
August 2024
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan.
Nanopore sensing is a label-free single-molecule technique that enables the study of the dynamical structural properties of proteins. Here, we detect the translocation of cytochrome (Cyt ) through an asymmetric thin nanopore with photothermal heating to evaluate the influence of temperature on Cyt conformation during its translocation in an electric field. Before Cyt translocates through an asymmetric thin SiN nanopore, ∼1 ms trapping events occur due to electric field-induced denaturation.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!