Electron spectroscopic imaging (ESI) of viruses using thin-section and immunolabelling preparations.

Ultramicroscopy

Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, Germany.

Published: January 1990

Electron spectroscopic imaging (ESI) and conventional bright-field transmission electron microscopy (TEM) were applied comparatively for the analysis of the fine structure and the antigenic make-up of human immunodeficiency virus and two herpes viruses. In addition to the information obtained in conventional bright-field TEM, ESI leads to high-contrast imaging of ultrathin sections and improves the resolution of thin and thick sections, and allows a better detectability of the immunolabelling markers.

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http://dx.doi.org/10.1016/0304-3991(90)90091-yDOI Listing

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