AI Article Synopsis
- The text discusses an autofocus algorithm for phase retrieval in phase contrast microscopy that reconstructs the phase of an exit wave function from just one image.
- The algorithm works without needing prior knowledge of the imaging system or the object being imaged, which is a significant advantage.
- It has been tested on electron micrographs of latex spheres, showing impressive accuracy with less than 10% RMS error across various defocus levels.
Article Abstract
Phase retrieval is widely used in phase contrast microscopy. Here we present an autofocus algorithm that allows the phase of the exit wave function, from a single-material object, to be reconstructed at medium resolution from a single phase contrast image without any a priori knowledge of the imaging system or object. The algorithm is demonstrated on coherent out-of-focus electron micrographs of 30 nm latex sphere calibration standards, giving <10% RMS error over a large defocus range.
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| http://dx.doi.org/10.1364/OL.36.001878 | DOI Listing |
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