We report the surface modification of microscopic live multicellular nematodes Caenorhabtidis elegans with polyelectrolyte multilayers (pure and doped with 20 nm gold nanoparticles) and the direct magnetic functionalization of nematodes with biocompatible magnetic nanoparticles. Magnetically functionalized "ironoxideclad" nematodes can be effectively separated and moved using an external magnetic field. The surface-functionalized nematodes preserve their viability and reproduction.
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http://dx.doi.org/10.1021/la2006869 | DOI Listing |
J Vis Exp
March 2022
Neuroscience Department, Wellesley College;
Caenorhabtidis elegans (C. elegans) is an optimal model organism for research and education at primarily undergraduate institutions. Undergraduates can quickly learn the sterile technique required to maintain C.
View Article and Find Full Text PDFInt J Mol Sci
December 2019
Laboratory of Marine Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Alameda 3363, Santiago 9170022, Chile.
In this work, transcripts encoding three metallothioneins from (UcMTs) were amplified: The 5'and 3' UTRs by RACE-PCR, and the open reading frames (ORFs) by PCR. Transcripts encoding UcMT1.1 (-like), UcMT2 (-like), and UcMT3 (-like) showed a 5'UTR of 61, 71, and 65 nucleotides and a 3'UTR of 418, 235, and 193 nucleotides, respectively.
View Article and Find Full Text PDFBiochem Biophys Rep
July 2017
Faculty of Health Sciences, Bacteriology and Clinical Laboratory Program, Colegio Mayor de Cundinamarca University, Calle 28 No. 5B-02, Bogotá, Colombia.
The NB327 mutant strain is characterized for the knockdown of the gene. The gene is homologous to the gene in humans, encoding the protein DICE-1 as a tumor suppressor. Absence or under-regulation of the gene can be reflected in lung and prostate cancer [17], [18].
View Article and Find Full Text PDFFront Plant Sci
May 2017
Division of Nematology, Indian Agricultural Research Institute (ICAR)New Delhi, India.
Plant parasitic nematodes cause severe damage and yield loss in major crops all over the world. Available control strategies include use of insecticides/nematicides but these have proved detrimental to the environment, while other strategies like crop rotation and resistant cultivars have serious limitations. This scenario provides an opportunity for the utilization of technological advances like RNA interference (RNAi) to engineer resistance against these devastating parasites.
View Article and Find Full Text PDFPLoS One
February 2012
Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, D. C., United States of America.
Parasitic hookworms and the free-living model nematode Caenorhabtidis elegans share a developmental arrested stage, called the dauer stage in C. elegans and the infective third-stage larva (L3) in hookworms. One of the key transcription factors that regulate entrance to and exit from developmental arrest is the forkhead transcription factor DAF-16/FoxO.
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