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Structure-switchable branched inhibitors regulate the activity of CRISPR-Cas12a for nucleic acid diagnostics.
Anal Chim Acta
January 2025
Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China; Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan, People's Republic of China; Hubei Engineering Center for Infectious Disease Prevention, Control and Treatment, Wuhan, People's Republic of China. Electronic address:
Background: In current years, the CRISPR (clustered regularly interspaced short palindromic repeats) based strategies have emerged as the most promising molecular tool in the field of gene editing, intracellular imaging, transcriptional regulation and biosensing. However, the recent CRISPR-based diagnostic technologies still require the incorporation of other amplification strategies (such as polymerase chain reaction) to improve the cis/trans cleavage activity of Cas12a, which complicates the detection workflow and lack of a uniform compatible system to respond to the target in one pot.
Results: To better fully-functioning CRISPR/Cas12a, we reported a novel technique for straightforward nucleic acid detection by incorporating enzyme-responsive steric hindrance-based branched inhibitors with CRISPR/AsCas12a methodology.
Enhancing Wear Resistance in Glass Ionomer Cement through Green-mediated Chitosan-, Titanium-, Zirconium-, and Hydroxyapatite-based Nanocomposites: An Analysis before and after Chewing Simulator Endurance.
Int J Clin Pediatr Dent
November 2024
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
Aim And Background: Glass ionomer cement (GIC) serves as a widely used restorative dental material, known for its direct bonding to tooth structures and fluoride-releasing properties. This study aims to investigate the enhancement of GIC through the incorporation of a green-mediated nanocomposite comprising chitosan, titanium, zirconium, and hydroxyapatite, with a focus on evaluating the wear resistance of the modified GIC.
Materials And Methods: A one-pot synthesis technique was utilized to prepare a green-mediated nanocomposite incorporating chitosan, titanium, zirconium, and hydroxyapatite nanoparticles.
An antimicrobial and adhesive conductive chitosan quaternary ammonium salt hydrogel dressing for combined electrical stimulation and photothermal treatment to promote wound healing.
Carbohydr Polym
March 2025
Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, School of Material Science and Engineering, Hubei University, Wuhan 430062, China. Electronic address:
The aim of this study is to investigate the effect of the adhesive, conductive hydrogel on wound healing when used as a therapeutic dressing. Herein, a dressing of PVA/QCS/TP@Fe (PQTF) was designed and prepared integrating polyvinyl alcohol (PVA), chitosan quaternary ammonium salt (QCS), tea polyphenol (TP), and ferric ions (Fe) by a simple one-pot and freeze-thaw method. In view of the comprehensive properties of PQTF hydrogel, including adhesion, electrical conductivity, and swelling performance, PQTF was selected for subsequent in vitro and in vivo healing promotion studies.
View Article and Find Full Text PDFSimple and Efficient Synthesis of -Succinimidyl-4-[F]fluorobenzoate ([F]SFB)-An Important Intermediate for the Introduction of Fluorine-18 into Complex Bioactive Compounds.
Pharmaceuticals (Basel)
December 2024
N. P. Bechtereva Institute of the Human Brain, Russian Academy of Sciences, 197022 St. Petersburg, Russia.
-succinimidyl-[F]fluorobenzoate ([F]SFB) is commonly prepared through a three-step procedure starting from [F]fluoride ion. A number of methods for the single-step radiosynthesis of [F]SFB have been introduced recently, including the radiofluorination of diaryliodonium salts and the Cu-mediated F-fluorination of pinacol aryl boronates and aryl tributyl stannanes, but they still have the drawbacks of lengthy product purification procedures. In the present work, two approaches for the direct labeling of [F]SFB from diaryliodonium (DAI) salt () and pinacol aryl boronate () are evaluated, with a major focus on developing a fast and simple SPE-based purification procedure.
View Article and Find Full Text PDFOne-Pot Synthesis of β-Alanine from Fumaric Acid via an Efficient Dual-Enzyme Cascade Biotransformation.
Biomolecules
December 2024
National Engineering Research Center of Wheat and Corn Further Processing, Henan University of Technology, Zhengzhou 450001, China.
As the only naturally occurring β-amino acid, β-alanine has important application prospects in many fields. Driven by the huge demand, biosynthesis is becoming more and more popular as a potential alternative to the chemical synthesis of β-alanine. Although the direct pathway from L-aspartic acid to β-alanine, catalyzed by L-aspartic acid-α-decarboxylase (PanD), is ideal for β-alanine synthesis, it is hindered by the high cost of the substrate and limited economic viability.
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