Fusarium graminearum from expression analysis to functional assays.

Methods Mol Biol

Department of Plant Biology, Michigan State University, East Lansing, MI, USA.

Published: September 2011

AI Article Synopsis

  • Fusarium graminearum is a key fungus responsible for wheat head blight and was the third filamentous fungus to have its genome fully sequenced in 2003.
  • This organism has emerged as a valuable model for various scientific studies, including genomics, mycotoxins, and fungal genetics.
  • The text discusses laboratory techniques used to analyze gene expression during different life cycle stages of F. graminearum and methods for functionally characterizing interesting genes.

Article Abstract

Fusarium graminearum, the causal agent of head blight of wheat, was the third filamentous fungus to have a completed genome sequence. Since the release of the genome sequence in 2003, F. graminearum has become a model for studies of genomics and transcriptomics, mycotoxins, fungal population genetics, gene function, and sexual development. Herein we present the techniques we have used in our laboratory to perform expression analyses on life cycle stages of F. graminearum and techniques to functionally characterize those genes identified as potentially interesting.

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http://dx.doi.org/10.1007/978-1-61779-040-9_6DOI Listing

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