AI Article Synopsis
- Adenoviral vector (AdV), specifically the third generation known as helper-dependent adenoviral vector (HDV), is promising for gene therapy but faces challenges in high-quality production.
- Recent advances have improved the large-scale manufacturing of HDV, allowing for greater volume and reduced production time.
- Efficient downstream processing techniques have been developed to ensure good recovery of HDV while minimizing contamination by the helper virus.
Article Abstract
Adenoviral vector (AdV) of the third generation also known as helper-dependent adenoviral vector (HDV) is an attractive delivery system for gene therapy applications. However, obtaining high quality-grade HDV in sufficient amount remains a challenge that hampers the extensive use of this vector in preclinical and clinical studies. Here we review recent progress in the large-scale manufacturing of HDV. The production of HDV is now amenable to large-scale volume with reduced process duration under optimized rescue and co-infection conditions. Also, efficient downstream processing of HDV with acceptable recovery of HDV and minimal contamination by the helper virus is described.
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| http://dx.doi.org/10.1007/978-1-61779-095-9_6 | DOI Listing |
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