Isolation and characterization of microsatellite markers in an endemic Hawaiian planthopper (Nesosydne chambersi: Delphacidae).

Mol Ecol Resour

Department of Environmental Science, Policy and Management, 137 Mulford Hall, University of California, Berkeley, Berkeley, CA 94720-3114, USA.

Published: November 2008

AI Article Synopsis

  • Researchers have identified and analyzed 17 microsatellite loci in the Hawaiian planthopper Nesosydne chambersi to study genetic variability.
  • The study included 30 individuals from one population and 10 individuals from two others, revealing two to nine alleles per locus.
  • These genetic markers will help explore population structure and intraspecific variation within this species.

Article Abstract

We have isolated and characterized 17 microsatellite loci for the endemic Hawaiian planthopper Nesosydne chambersi (Delphacidae), a member of a large Hawaiian Nesosydne radiation. Thirty individuals from one population and 10 individuals from two populations across the species' range were tested to investigate polymorphism. The observed loci contained two to nine alleles per locus. Expected heterozygosity within this species ranged from 0.2 to 0.85. These markers will be used to assess intraspecific differentiation and population structure within N. chambersi.

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Source
http://dx.doi.org/10.1111/j.1755-0998.2008.02253.xDOI Listing

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