Improved efficacy of prebiotic by flaxseed oil and horse chestnut in experimental colon cancer.

Objectives: This experimental work was designed to investigate the efficacy of prebiotic by itself and in combination with Hyppocastani extractum siccum, and Lini oleum virginale on selected parameters in rats with dimethylhydrazine induced colon cancer.

Methods: Rats were randomly divided into 5 experimental groups of 12 rats each. Rats were fed with high fat (HF) diet containing 10 % of fat, supplemented by prebiotic at a dose of 2 % of HF diet itself and in combination with Hyppocastani extractum siccum at a dose of 1 % of diet and Lini oleum virginale at a dose of 2 % of diet. Two weeks after the start of the diet dimethylhydrazine injections in dose 20 mg/kg b.w. were applied (DMH, Merck, DE), two times at week interval. The activity of beta-glucuronidase, concentration of lipid parameters, bile acids and short chain fatty acids were determined.

Results: Prebiotic and its combinations with selected substances significantly decreased the activity of bacterial enzyme beta-glucuronidase (p<0.001). Bile acids concentration was significantly decreased (p<0.01) excepting combination of prebiotic with Horse chestnut. Self applied prebiotic decreased (p<0.001) lipids parameters (total cholesterol and triacylglycerols), and enhanced short chain fatty acids production.

Conclusion: Prebiotics have protective effect and may be the useful candidate agents for colon cancer prevention and treatment. The application of selected bioactive food components supported the effect of prebiotics (Tab. 2, Fig. 1, Ref. 16). Full Text in free PDF www.bmj.sk.