The high separating speed, efficiency and operational stability of various micropellicular stationary phases are demonstrated in the high-performance liquid chromatography (HPLC) of biopolymers. The micropellicular sorbents were prepared from 2-microns fluid-impervious silica microspheres as the support, with a thin layer of different retentive materials at the surface. These include a molecular fur of octyl or stearyl chains for reversed-phase chromatography as well as a hydrophilic layer with amino groups and polyethyleneglycol chains for anion-exchange and hydrophobic interaction chromatography, respectively. The use of appropriate micropellicular stationary phases for protein separation by metal-interaction and affinity chromatography is also illustrated. In most cases, operation at elevated column temperature was found to be preferable for rapid separations. Preliminary results show that the stability of micropellicular columns compares very favorably with that of columns conventionally used in HPLC and that they are easy to maintain.
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