Objective: To examine the in vivo visual imaging of buccal carcinoma with the near-infrared fluorescent quantum dots.
Methods: The U14 cells were labeled by endocytosis with QD800 (U14/QD800) which was linked with cell-penetrating peptide. Different number of U14/QD800 was injected under the buccal mucosa of nude mice and Kunming mice separately and imaged at different time to detect the in vivo sensitivity and dynamic imaging of U14/QD800.
Results: The minimum number of U14/QD800 cells which could be detected by in vivo imaging system was 1 × 10(4) in nude mice's cheek and 1 × 10(5) in Kunming mice's. The time for visual imaging of 1 × 10(4), 1 × 10(5) and 1 × 10(6) U14/QD800 cells in nude mice was 3, 7 and 16 d separately, and 3 and 10 d separately in Kunming mice.
Conclusions: Due to its strong tissue penetration, near-infrared fluorescent quantum dots have great prospects in cancer early diagnosis, visual observation and individual treatment.
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