[The curative effects of bone mesenchymal stem cells transplanted into rats with Sjogren's syndrome].

Purpose: To study the curative effects, survival rate, migration and differentiation of bone mesenchymal stem cells (BMSCs) transplanted into rats with Sjogren's syndrome.

Methods: Model of Sjogren's syndrome was created in rats. BMSCs were isolated and cultured by using the preceding method. Then the pretreated BMSCs were identified and labeled by enhanced green fluorescent protein. BMSCs marked with enhanced green fluorescent protein (EGFP) or PBS were injected into the SMG of the Sjogren's syndrome rats via open surgery.Total static saliva flow rate was determined in rats. The daily amount of water in the normal group, the model group, the model treatment group and the model treatment control group was recorded. The survival rate, migration and differentiation of the BMSCs transplanted in the treatment group under fluorescence microscope was recorded. Student's t test was used for data analysis using SPSS 12.0 software package.

Results: Compared with the model treatment control group, the total static saliva flow rate of the model treatment group increased significantly, and the water they drank decreased significantly (P<0.05). In addition, BMSCs were distributed along the injection tract mostly in the first week,then BMSCs were mainly distributed in the stroma between the acinar in the second week and were distributed over other areas four weeks later. Immunohistochemical staining of amylzyme was not observed at the first week after transplantation. And at the 8th week the expression of amylzyme in the cytoplasm of the transplanted BMSCs was observed by immunohistochemical only in the model treatment group.

Conclusion: Transplantation of BMSCs has certain treatment effect on Sjogren's syndrome.