Building a global library of DNA barcodes will require efficient logistics of pre-laboratory specimen processing and seamless interfacing with molecular protocols. If not addressed properly, the task of aggregating specimens may become the biggest bottleneck in the analytical chain. Three years of experience in developing a collection management system to facilitate high-throughput DNA barcoding have allowed the Canadian Centre for DNA Barcoding to recognize and resolve the most common logistical obstacles. Dealing with these challenges on a larger scale will be an important step towards building a solid collection-based foundation for the international DNA barcoding effort.
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http://dx.doi.org/10.1111/j.1755-0998.2009.02629.x | DOI Listing |
PLoS One
January 2025
Danau Girang Field Centre, c/o Sabah Wildlife Department, Kota Kinabalu, Malaysia.
Characterizing the feeding ecology of threatened species is essential to establish appropriate conservation strategies. We focused our study on the proboscis monkey (Nasalis larvatus), an endangered primate species which is endemic to the island of Borneo. Our survey was conducted in the Lower Kinabatangan Wildlife Sanctuary (LKWS), a riverine protected area that is surrounded by oil palm plantations.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Oregon Health & Science University, Portland, OR, USA.
Background: Single-cell technologies have revealed significant microglial cell heterogeneity across the human brain in both health and disease. However, the integration of high-plex protein and spatial information in single-cell approaches constitutes a challenge essential for advancing our cell biology comprehension in the neuroscience field.
Method: In the present study, we employed co-detection by indexing (CODEX), a protein multiplexed imaging technology, for the first time to unravel the association between different microglial populations and pathological features of Alzheimer's disease (AD) in the human brain.
Alzheimers Dement
December 2024
Sant Pau Memory Unit, Hospital de la Santa Creu i Sant Pau - Biomedical Research Institute Sant Pau - Universitat Autònoma de Barcelona, Barcelona, Spain.
Background: Synaptic degeneration is a primary neuropathological factor associated with cognitive decline in Alzheimer's disease (AD). In 2021, we generated a synaptic Polygenic Risk Score (PRS) that comprised only 8 variants within 6 synaptic genes (APOE, PICALM, BIN1, PTK2B, DLG2 and MINK1) that predicted AD with 72% accuracy in two neuropathological cohorts. This supports the hypothesis that genetic variants that regulate an individual's vulnerability to AD-related synapse degeneration could be used to identify individuals at-risk for AD prior to the appearance of clinical symptoms.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Stem Cell Program, Boston Children's Hospital, Boston, MA, USA.
The CRISPR-activated repair lineage tracing (CARLIN) mouse line uses DNA barcoding to enable high-resolution tracing of cell lineages in vivo (Bowling et al, Cell 181, 1410-1422.e27, 2020). CARLIN mice contain expressed barcodes that allow simultaneous interrogation of lineage and gene expression information from single cells.
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