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Isolation and characterization of microsatellite loci for storm-petrels. | LitMetric

Isolation and characterization of microsatellite loci for storm-petrels.

Mol Ecol Resour

Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6 Departament Biologia Animal, Universitat de Barcelona, Avenue Diagonal 645, 08028 Barcelona, Spain.

Published: May 2009

AI Article Synopsis

  • Primers for 10 microsatellite loci were created for two species of Oceanodroma storm-petrels (O. castro and O. monteiroi) and tested for genetic variability.
  • At least six loci were consistently amplifiable and showed genetic diversity in each species, with an average of 4.6 alleles per locus and 0.41 average heterozygosity.
  • The primers also produced results in additional related species, indicating their potential for studying population genetic structure in storm-petrels.

Article Abstract

Primers were developed for 10 microsatellite loci for two species of Oceanodroma storm-petrels. Variability was tested in 27 O. castro and 22 O. monteiroi from the Azores, and 24 O. leucorhoa from Norway. At least six loci amplified reliably and were polymorphic in each species. The number of alleles per locus averaged 4.6, and observed heterozygosities averaged 0.41. Most primers also yielded polymerase chain reaction products in O. tethys, O. hornbyi and Pterodroma phaeopygia. These loci are being used to assay population genetic structure in storm-petrels.

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Source
http://dx.doi.org/10.1111/j.1755-0998.2008.02487.xDOI Listing

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