Thirty polymorphic microsatellite loci were developed from the critically endangered kakapo (Strigops habroptilus), using an enriched genomic library. Characterization of loci using 90 kakapo revealed an average of 3.3 alleles per locus (range: 2-5) and an average expected heterozygosity of 0.47 (range: 0.17-0.70). The probability of identity (7.2 × 10(-15) ) and probability of exclusion (0.999999) demonstrate that these loci are a highly informative marker set that can aid the genetic management of the kakapo.
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http://dx.doi.org/10.1111/j.1755-0998.2008.02506.x | DOI Listing |
Mol Genet Genomics
December 2024
Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, Henan, China.
Given the high morbidity, mortality, and hereditary risk of cardiovascular diseases (CVDs), their prevention and control have garnered widespread attention and remain central to clinical research. This study aims to assess the feasibility and necessity of haplotyping-based preimplantation genetic testing for the prevention of inherited CVD. A total of 15 preimplantation genetic testing for monogenic defect (PGT-M) cycles were performed in 12 CVD families from January 2016 to July 2022.
View Article and Find Full Text PDFSci Rep
December 2024
Hy-Line International, 2583 240th St, PO Box 310, Dallas Center, 50063, IA, USA.
Marek's Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F population, and by selective DNA pooling (SDP) of 8 elite egg production lines.
View Article and Find Full Text PDFXenobiotica
December 2024
Department of Pharmacy, Akita University Hospital, Akita, Japan.
The aim of this study was to investigate the effects of renal function and polymorphism on the drug interaction between venetoclax and fluconazole in thirty acute myeloid leukaemia patients.The area under the plasma concentration-time curve (AUC) and trough concentration (C) of venetoclax and the fluconazole C were obtained from plasma samples on day 7 later after initiation of venetoclax 200 mg/day combined with fluconazole.The fluconazole C values in patients with moderate and severe renal impairment were significantly higher than those in patients with normal or mild impairment (median values 7037, 6234, and 4813 ng/mL, respectively, = 0.
View Article and Find Full Text PDFISME J
December 2024
Université Paris Cité, INSERM, IAME, 75018 Paris, France.
Escherichia coli is an increasingly antibiotic-resistant opportunistic pathogen. Few data are available on its ecological and evolutionary dynamics in its primary commensal niche, the vertebrate gut. Using Illumina and/or Nanopore technologies, we sequenced whole genomes of 210 E.
View Article and Find Full Text PDFMedicine (Baltimore)
December 2024
General Medicine Department, Emergency General Hospital, Beijing, China.
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