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Isolation and characterization of polymorphic microsatellite loci from Areca catechu (Arecaceae) using PCR-based isolation of microsatellite arrays. | LitMetric

Isolation and characterization of polymorphic microsatellite loci from Areca catechu (Arecaceae) using PCR-based isolation of microsatellite arrays.

Mol Ecol Resour

Institute of Anthropology, National Tsing Hua University, Hsinchu, Taiwan 300 Department of Life Sciences, Cheng Kung University, Tainan, Taiwan 701 Taiwan Endemic Species Research Institute, Nantou, Taiwan 552.

Published: March 2009

AI Article Synopsis

  • Betel nut, a plant native to tropical and subtropical regions, plays a significant role in the cultures of Southeast Asia and Oceania and is linked to human migration patterns important in ethnobotany and anthropology.
  • The study explored nine microsatellite loci from the betel nut plant, revealing a range of 5 to 15 alleles per locus, with varying levels of expected and observed heterozygosities.
  • Most microsatellite loci showed deviations from Hardy-Weinberg equilibrium, likely due to selective cultivation or null alleles, while no linkage disequilibrium was found, except between two specific loci (AC06 and AC08).

Article Abstract

Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropology. In this study, we described the development of nine microsatellite loci from A. catechu. The number of alleles per locus ranged from 5 to 15. The expected and observed heterozygosities ranged from 0.71to 0.94 and from 0 to 0.88, respectively. All microsatellite loci, except for AC30, significantly deviated from Hardy-Weinberg equilibrium possibly due to artificially selected cultivation or the existence of excessive null alleles. No linkage disequilibrium was observed from pairwise comparisons of loci, except for AC06 and AC08.

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Source
http://dx.doi.org/10.1111/j.1755-0998.2008.02504.xDOI Listing

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