Isolation and characterization of microsatellite markers from the great hornbill, Buceros bicornis.

Mol Ecol Resour

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand Department of Zoology, National Museum of Nature and Science, Tokyo 169-0073, Japan Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Published: March 2009

AI Article Synopsis

  • Thirteen polymorphic microsatellite markers were identified and analyzed in the great hornbill species, Buceros bicornis.
  • In a study of 20 individual birds, the number of alleles at each microsatellite locus ranged from 2 to 11, with varying levels of expected and observed heterozygosities.
  • The markers are valuable for further population genetic research on the great hornbill, despite three loci showing deviations from Hardy-Weinberg equilibrium.

Article Abstract

Thirteen polymorphic microsatellite markers were isolated and characterized from the great hornbill, Buceros bicornis. In analyses of 20 individuals, the numbers of alleles per locus varied from two to 11. The expected and observed heterozygosities ranged from 0.22 to 0.88 and from 0.20 to 1.00, respectively. The mean polymorphic information content was 0.62. Among these, three loci deviated from the Hardy-Weinberg equilibrium. However, no significant genotypic disequilibrium was detected between any pair of loci. These microsatellite markers are useful for the population genetic study of the great hornbill.

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Source
http://dx.doi.org/10.1111/j.1755-0998.2008.02447.xDOI Listing

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