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Analysis of differential gene expression of PBMC for the in vitro detection of drug sensitization.
Allergol Int
January 2025
Research Division, Federal Institute for Drugs and Medical Devices (BfArM), Bonn, Germany; Department of Dermatology and Allergy, University Hospital Aachen, Aachen, Germany.
Background: The detection of drug-specific activation of T cells in the lymphocyte transformation test (LTT) is mainly based on cell proliferation or cytokine secretion. However, the LTT presents with a varying sensitivity and specificity. The aim of our study was to analyse the genome wide gene expression of PBMC to identify drug allergy-specific gene regulation patterns.
View Article and Find Full Text PDFEnhanced nano-LC-MS for analyzing dansylated oral cancer tissue metabolome dissolved in solvents with high elution strength.
Anal Chim Acta
February 2025
Department of Biochemistry and Molecular Biology, Chang Gung University, Taoyuan, 333, Taiwan; Clinical Proteomics Core Laboratory, LinKou Chang Gung Memorial Hospital, Taoyuan, 333423, Taiwan. Electronic address:
Background: Tissue metabolomics analysis, alongside genomics and proteomics, offers crucial insights into the regulatory mechanisms of tumorigenesis. To enhance metabolite detection sensitivity, chemical isotope labeling (CIL) techniques, such as dansylation, have been developed to improve metabolite separation and ionization in mass spectrometry (MS). However, the dissolution of hydrophobic derivatized metabolites in solvents with high acetonitrile content limits the use of liquid chromatography (LC) systems with small-volume reversed-phase (RP) columns.
View Article and Find Full Text PDFBiarsenical-based fluorescent labeling of metallothioneins as a method for ultrasensitive quantification of poly-Cys targets.
Anal Chim Acta
February 2025
Department of Chemical Biology, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, 50-383, Wrocław, Poland. Electronic address:
Background: Mammalian metallothioneins (MTs) play a crucial role in maintaining Zn(II) and Cu(I) homeostasis, as well as regulating the cellular redox potential. They are involved in cancer resistance to cisplatin-related drugs and the sequestration of toxic metal ions. To investigate their participation in specific physiological and pathological processes, it is imperative to develop an analytical method for measuring changes in protein concentration both in vitro and in vivo.
View Article and Find Full Text PDFFrontiers in laccase nanozymes-enabled colorimetric sensing: A review.
Anal Chim Acta
February 2025
Nanobiophotonics Department, Leibniz Institute of Photonic Technology (Leibniz-IPHT), Albert-Einstein-Strasse 9, 07745, Jena, Germany. Electronic address:
In recent years, nanozyme-based analytics have become popular. Among these, laccase nanozyme-based colorimetric sensors have emerged as simple and rapid colorimetric detection methods for various analytes, effectively addressing natural enzymes' stability and high-cost limitations. Laccase nanozymes are nanomaterials that exhibit inherent laccase enzyme-like activity.
View Article and Find Full Text PDFSelf-powered photoelectrochemical sensor based on molecularly imprinted polymer-coupled CBFO photocathode and AgS/SnS photoanode for ultrasensitive dimethoate sensing.
Anal Chim Acta
February 2025
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China. Electronic address:
Dimethoate (DIM) is one of the most extensively applied organophosphorus pesticides (OPs), which is used to boost farm productivity due to its high insecticidal efficacy. However, the excessive use of DIM can result in the extensive contamination of soil, groundwater and food. Monitoring of DIM in environmental and food samples is crucial in view of its potential health risks and environmental hazards from excessive residues.
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