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Probing the interactions of an acyl carrier protein domain from the 6-deoxyerythronolide B synthase. | LitMetric

AI Article Synopsis

  • The architecture of polyketide synthases (PKSs) allows for the potential reprogramming of antibiotic production by manipulating biosynthetic pathways.* -
  • A key challenge is understanding how reactive intermediates move efficiently through the PKS assembly line, particularly the role of the acyl carrier protein (ACP) in interacting with different enzyme domains.* -
  • Through NMR spectroscopy, researchers identified important protein-protein interactions and the influence of acyl chain substrates, developing a new probe to enhance the study of these interactions and advance understanding of chain translocation.*

Article Abstract

The assembly-line architecture of polyketide synthases (PKSs) provides an opportunity to rationally reprogram polyketide biosynthetic pathways to produce novel antibiotics. A fundamental challenge toward this goal is to identify the factors that control the unidirectional channeling of reactive biosynthetic intermediates through these enzymatic assembly lines. Within the catalytic cycle of every PKS module, the acyl carrier protein (ACP) first collaborates with the ketosynthase (KS) domain of the paired subunit in its own homodimeric module so as to elongate the growing polyketide chain and then with the KS domain of the next module to translocate the newly elongated polyketide chain. Using NMR spectroscopy, we investigated the features of a structurally characterized ACP domain of the 6-deoxyerythronolide B synthase that contribute to its association with its KS translocation partner. Not only were we able to visualize selective protein-protein interactions between the two partners, but also we detected a significant influence of the acyl chain substrate on this interaction. A novel reagent, CF₃-S-ACP, was developed as a ¹⁹F NMR spectroscopic probe of protein-protein interactions. The implications of our findings for understanding intermodular chain translocation are discussed.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149197PMC
http://dx.doi.org/10.1002/pro.652DOI Listing

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