When normal human fibroblast cells (MRC-5) received a priming irradiation of 3-20 mGy 4 h prior to irradiation with 1000 mGy, the number of DNA double-stranded breaks (DSBs) decreased significantly to 18.2-18.7 per cell compared with 21 per cell when there was no priming irradiation. This result indicates that a priming irradiation of 3-20 mGy induces a radioadaptive response in MRC-5. The authors' previous study had indicated that DSBs induced by ≤ 20 mGy are due to a radiation-induced bystander effect. These findings suggest that radiation-induced bystander effects might contribute to induction of the radioadaptive response. To test this hypothesis, MRC-5 were suspended in lindane, an inhibitor of radiation-induced bystander effects, which was added to the medium for the priming irradiation of 3-20 mGy. Lindane inhibited the protective effect of priming irradiation on DSBs caused by subsequent irradiation with 1000 mGy. Thus, radiation-induced bystander effects may play a role in radioadaptive responses.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/rpd/ncr169 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
STUDY OF CHROMOSOME STABILITY OF HUMAN SOMATIC CELLS IN THE DEVELOPMENT OF THE SECONDARY RADIATION-INDUCED BYSTANDER EFFECT.
Probl Radiac Med Radiobiol
December 2024
State Institution «National Research Center of Radiation Medicine, Hematology and Oncology of the National Academy of Medical Sciences of Ukraine», 53 Yuriia Illienka Str., Kyiv, 04050, Ukraine.
Objective: To establish the level of chromosomal instability in human peripheral blood lymphocytes during thedevelopment of secondary radiation-induced bystander effect.
Materials And Methods: Human peripheral blood lymphocytes; culture of human non-small-cell lung cancer cell lineA549 (irradiated in vitro by 137Cs in a dose of 0.50 Gy/unirradiated).
Radiation-induced Bystander Effect in Starfish (Patiria pectinifera) Oocytes.
Radiat Res
January 2025
Integrated Center for Science and Humanities, Fukushima Medical University, Fukushima, 960-8516, Japan.
Although multiple studies suggest that ionizing radiation can induce bystander effects (radiation-induced bystander effect, RIBE) in cultured cell lines, it is still unclear whether RIBE is evolutionarily conserved in invertebrates. In this study, we investigated the frequency of cell death of unirradiated starfish (Patiria pectinifera) oocytes co-cultured with oocytes irradiated with X rays (0, 2 and 4 Gy). We observed increased frequencies of cell death determined by morphological abnormality and TUNEL-positive cells in unirradiated oocytes co-cultured with oocytes irradiated with 2 Gy or 4 Gy oocytes.
View Article and Find Full Text PDFRole of damaged mitochondrial transfer in alpha-particle generator Pb radiation-induced bystander effect.
Theranostics
October 2024
Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Pb, a promising alphaparticle generator of Bi, has aroused much interest as a therapeutic radionuclide. For the development of targeted alpha therapy (TAT), it is important to determine the contribution of targeted effects in irradiated cells, and also of non-targeted effects in non-irradiated bystander cells. Currently, the critical roles of mitochondrial transfer in cellular crosstalk have garnered significant attention.
View Article and Find Full Text PDFRadiation-Induced Macrovessel/Microvessel Disease.
Arterioscler Thromb Vasc Biol
December 2024
Free Radical and Radiation Biology Program, Department of Radiation Oncology (B.G.A., K.A.M., I.M.G.), Carver College of Medicine, University of Iowa.
Radiation therapy (RT) is a cornerstone in cancer treatment (used in 50% of cases), yet challenges persist because damage to normal tissue through direct impact of radiation or bystander effects is inevitable. Injury of macrovessels by RT manifests as obstructive disease, which is akin to atherosclerotic disease. Historically observed in coronary arteries of patients treated for breast cancer and lymphoma, it also affects patients receiving contemporary therapy for lung and chest cancers.
View Article and Find Full Text PDFMultidimensional screening of Astragalus membranaceus small molecules to mitigate carbon ion radiation-induced bystander effects.
J Pharm Anal
October 2024
Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou, 73000, China.
Article Synopsis
- Existing studies show that compounds from Astragalus membranaceus (AM) help reduce radiation damage, but there hasn't been research on how isoliquiritigenin (ISL) affects bone marrow stem cells in carbon ion radiation therapy for lung cancer.
- This study aimed to explore ISL's ability to enhance the effectiveness of radiotherapy while protecting stem cells from damage, using various experimental methods and models.
- The findings indicate that ISL significantly improves the anti-tumor effects of carbon ion radiotherapy and decreases radiation harm to bone marrow stem cells, suggesting its potential as a radiosensitizer in lung cancer treatment.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!