AI Article Synopsis
- A new method has been developed to measure salicylic acid and salicylamide in biological fluids, overcoming challenges due to their overlapping fluorescence spectra.
- The technique utilizes fluorescence decay curves, which enhance selectivity and sensitivity compared to traditional methods.
- Tests with synthetic samples showed recovery rates between 98.2% and 104.6%, and the method also demonstrated effective detection limits in human serum and urine, with recoveries of 96.2% for salicylic acid and 94.5% for salicylamide.
Article Abstract
A new methodology for the simultaneous determination of salicylic acid and salicylamide in biological fluids is proposed. The strong overlapping of the fluorescence spectra of both analytes makes impossible the conventional fluorimetric determination. For that reason, the use of fluorescence decay curves to resolve mixtures of analytes is proposed; this is a novel technique that provides the benefits in selectivity and sensitivity of the fluorescence decay curves. In order to assess the goodness of the proposed method, a prediction set of synthetic samples were analyzed obtaining recuperation percentages between 98.2 and 104.6%. Finally, a study of the detection limits was done using a new criterion resulting in values for the detection limits of 8.2 and 11.6 μg L(-1) for salicylic acid and salicylamide respectively. The validity of the method was tested in human serum and human urine spiked with aliquots of the analytes. Recoveries obtained were 96.2 and 94.5% for salicylic acid and salicylamide respectively.
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| http://dx.doi.org/10.1016/j.saa.2011.03.047 | DOI Listing |
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