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COUNTERPOINT: Should Transbronchial Cryobiopsies Be Considered the Initial Biopsy of Choice in Patients With a Possible Interstitial Lung Disease? No.
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Division of Pulmonary and Critical Care, Johns Hopkins University, Baltimore, MD.
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The effects of vitamin D on osteoblast mineralization are well documented. Reports of the effects of vitamin D on osteoclasts, however, are conflicting, showing both inhibition and stimulation. Finding that resorbing osteoclasts in human bone express vitamin D receptor (VDR), we examined their response to different concentrations of 25-hydroxy vitamin D [25(OH)D] (100 or 500 nmolL) and 1,25-dihydroxy vitamin D [1,25(OH)D] (0.
View Article and Find Full Text PDF[Expression and tumoricidal activity of a human-mouse chimeric anti-DR5 antibody through a Furin/2A peptide].
Zhonghua Yi Xue Za Zhi
March 2011
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
Objective: To express a full-length human-mouse chimeric anti-DR5 antibody from a single open reading frame with tumoricidal activity to various cancer cells.
Methods: The heavy and light chains of chimeric antibody were joined by the Furin and 2A (F/2A) self-cleavage peptide and cloned into a lentiviral vector of pWPXL. Then the HEK293 cells were infected with the constructed expression vector pWPXL-HF2AL.
Counterpoint: Both immunohistochemistry and fluorescence in situ hybridization play important roles for HER2 evaluation.
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