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| http://dx.doi.org/10.1128/AAC.01634-10 | DOI Listing |
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Biomimetic exosome harnessing exosomal lipidomics and functional proteins for PEDF-pDNA delivery in high altitude pulmonary edema intervention.
J Control Release
January 2025
School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu Province, China. Electronic address:
In the realm of gene therapy, given the exceptional performance of native exosomes, researchers have redirected their innovative focus towards exosome-mimetic nanovesicles (EMNs); however, the current design of most EMNs relies heavily on native cells or their components, inevitably introducing inter-batch variability issues and posing significant challenges for quality control. To overcome the excessive reliance on native cellular components, this study adopts a unique approach by precisely mimicking the lipid composition of exosomes and innovatively incorporating histone components to recapitulate the gene transfer characteristics of exosomes. We selected sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylethanolamine (PE), and cholesterol as the lipid components, and employed the double emulsion method to prepare biomimetic exosomes carrying histone A and PEDF-DNA plasmids (His-pDNA@EMNs).
View Article and Find Full Text PDFSynthesis and Investigation of Peptide-Drug Conjugates Comprising Camptothecin and a Human Protein-Derived Cell-Penetrating Peptide.
Chem Biol Drug Des
January 2025
Research School of Chemistry, Australian National University, Canberra, Australian Capital Territory, Australia.
Drug targeting strategies, such as peptide-drug conjugates (PDCs), have arisen to combat the issue of off-target toxicity that is commonly associated with chemotherapeutic small molecule drugs. Here we investigated the ability of PDCs comprising a human protein-derived cell-penetrating peptide-platelet factor 4-derived internalization peptide (PDIP)-as a targeting strategy to improve the selectivity of camptothecin (CPT), a topoisomerase I inhibitor that suffers from off-target toxicity. The intranuclear target of CPT allowed exploration of PDC design features required for optimal potency.
View Article and Find Full Text PDFBoosting porphyrin synthesis and ALA-mediated photoinactivation through near-infrared therapy.
Photochem Photobiol
January 2025
Laboratorio de Terapias Fotoasistidas, Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Hospital de Clínicas José de San Martín and CONICET, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.
Photodynamic inactivation (PDI) combines the use of photosensitizers with visible light to produce reactive oxygen species that effectively eliminate pathogens. To investigate the impact of near- infrared therapy (NIRT) on heme biosynthesis and permeability of the pro-photosensitizers 5-aminolevulinic acid (ALA) and Hexyl-ALA (H-ALA) through biofilms, we applied sub-lethal conditions for both NIRT and PDI to maintain intact bacterial viability. During NIRT, the temperature remained below 37°C, permitting rapid heating (ΔT = 11°C) without causing thermal damage.
View Article and Find Full Text PDF3D fluorescence staining and confocal imaging of low amount of intestinal organoids (enteroids): Protocol accessible to all.
PLoS One
January 2025
Laboratory of Hepato-Gastroenterology, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
The emerging field of 3D organ modeling encounters several imaging issues in particular related to antigen retrieval and sample loss during staining processes. Due to their compact shape, several antibodies fail to penetrate intact organoids or spheroids. Histology of organoids can be approached by paraffin inclusion and sectioning at 5 μm as performed for biopsies.
View Article and Find Full Text PDFCaffeine as an Active Molecule in Cosmetic Products for Hair Loss: Its Mechanisms of Action in the Context of Hair Physiology and Pathology.
Molecules
January 2025
Department of Experimental Dermatology and Cosmetology, Jagiellonian University Medical College, ul. Medyczna 9, 30-688 Krakow, Poland.
Caffeine has recently attracted attention as a potential remedy for hair loss. In the present review, we look into the molecule's possible mechanisms of action and pharmacodynamics. At the molecular level, it appears that the physiological effects of caffeine are mainly due to the molecule's interaction with adenosine pathways which leads to an increase in cAMP level and the stimulation of metabolic activity in the hair follicle.
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