AI Article Synopsis
- The study investigates how asiaticoside affects the growth and behavior of fibroblasts related to capsular contracture after breast augmentation.
- Results showed that higher concentrations of asiaticoside significantly reduced DNA and collagen synthesis in fibroblasts, particularly noted at 50 mg/L, indicating it inhibits cell activity.
- Additionally, asiaticoside at 25 mg/L decreased the expression of alpha-SMA, a marker of fibroblast transformation into myofibroblasts, suggesting it helps prevent this conversion.
Article Abstract
Objective: To explore the cellular and molecular mechanism of the inhibitory effect of asiaticoside on capsular contracture following breast augmentation.
Methods: Contracture capsule derived fibroblasts were cultured in medium with different concentration of asiaticoside. The cell proliferation, collage synthesis and alpha-SMA expression were detected by means of 3H-thymidine incorporation, 3H-proline incorporation, and Western-blot. The results were analyzed by SPSS 11.0 with t test.
Results: DNA and collagen synthesis of fibroblasts were dramatically inhibited when the asiaticoside reached the concentration of 50 mg/L. The inhibitory rate was 34.7% and 30.1% respectively, showing a significant difference from that in control group (P < 0.05). The inhibitory effect increased with the rise of the asiaticoside concentration in a dose-dependent manner. When the concentration of asiaticoside reached 25 mg/L, the expression of alpha-SMA was down-regulated with an activation index of 1.673, showing a significant difference when compared with that in control group (P < 0.05).
Conclusions: asiaticoside can effectively inhibit the DNA and collagen synthesis of capsule-derived fibroblasts. The trans-differentiation of fibroblast to myo-fibroblasts is also prevented by it.
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