In this study, seven recombinant epitope peptides from within the ORF2 protein of the local genotype 4 swine hepatitis E virus (HEV) DQ strain were designed and analyzed. Then, a new multi-epitope-based ELISA was established. In comparison with a commercial kit, this test exhibited good specificity and sensitivity for anti-HEV genotype 4. Subsequently, this test was applied for analyzing serum samples from either swine herds or human populations in northern China. The overall seroprevalence rate of anti-HEV IgG reached up to 40.4% for swine and 8.1% for humans. A statistical difference was observed for humans in rural and urban areas, with a higher prevalence for people living in rural than urban areas. Moreover, sequencing confirmed that all RNA-positive samples belonged to genotype 4.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00705-011-1007-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Multi-Epitope Fusion Protein-Based p-ELISA Method for Diagnosing Bovine and Goat Brucellosis.
Front Vet Sci
September 2021
Key Lab of Environment and Health, School of Public Health, Xuzhou Medical University, Xuzhou, China.
In recent years, the incidence of brucellosis has increased annually, causing tremendous economic losses to animal husbandry in a lot of countries. Therefore, developing rapid, sensitive, and specific diagnostic techniques is critical to control the spread of brucellosis. In this study, bioinformatics technology was used to predict the B cell epitopes of the main outer membrane proteins of , and the diagnostic efficacy of each epitope was verified by an indirect enzyme-linked immunosorbent assay (iELISA).
View Article and Find Full Text PDFMulti-Epitope-Based Vaccines for Colon Cancer Treatment and Prevention.
Front Immunol
December 2021
University of Washington (UW) Medicine, Cancer Vaccine Institute, University of Washington, Seattle, WA, United States.
Article Synopsis
- Researchers investigated if overexpressed proteins linked to colorectal cancer could be used as effective vaccine targets to prevent tumor development in certain mouse models.
- They identified several antigens (CDC25B, COX2, RCAS1, FASCIN1) that showed higher immune responses in colorectal cancer patients, indicating their potential as vaccine candidates.
- Experiments showed that vaccination with CDC25B or COX2 peptides significantly reduced tumor growth in mice, while RCAS1 did not demonstrate any anti-tumor effect, highlighting the potential of these antigens in cancer immunotherapy.
Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques.
View Article and Find Full Text PDFIdentification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8.
Mol Med Rep
August 2015
Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
T-cell-mediated immunotherapy of hematological malignancies requires selection of targeted tumor-associated antigens and T-cell epitopes contained in these tumor proteins. Epidermal growth factor receptor pathway substrate 8 (EPS8), whose function is pivotal for tumor proliferation, progression and metastasis, has been found to be overexpressed in most human tumor types, while its expression in normal tissue is low. The aim of the present study was to identify human leukemia antigen (HLA)-A*0201-restricted epitopes of EPS8 by using a reverse immunology approach.
View Article and Find Full Text PDFChimerically fused antigen rich of overlapped epitopes from latent membrane protein 2 (LMP2) of Epstein-Barr virus as a potential vaccine and diagnostic agent.
Cell Mol Immunol
July 2016
Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, China.
Epstein-Barr virus (EBV) is prevalent throughout the world and is associated with several malignant diseases in humans. Latent membrane protein 2 (LMP2) of EBV plays a crucial role in the pathogenesis of EBV-associated tumors; therefore, LMP2 has been considered to be a potential immunodiagnostic and immunotherapeutic target. A multi-epitope-based antigen is a promising option for therapeutic vaccines and diagnoses of such malignancies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!