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Structural analysis of full-length Hfq from Escherichia coli. | LitMetric

Structural analysis of full-length Hfq from Escherichia coli.

Acta Crystallogr Sect F Struct Biol Cryst Commun

Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Campus Vienna Biocenter 5, A-1030 Vienna, Austria.

Published: May 2011

AI Article Synopsis

Article Abstract

The structure of full-length host factor Qβ (Hfq) from Escherichia coli obtained from a crystal belonging to space group P1, with unit-cell parameters a = 61.91, b = 62.15, c = 81.26 Å, α = 78.6, β = 86.2, γ = 59.9°, was solved by molecular replacement to a resolution of 2.85 Å and refined to R(work) and R(free) values of 20.7% and 25.0%, respectively. Hfq from E. coli has previously been crystallized and the structure has been solved for the N-terminal 72 amino acids, which cover ~65% of the full-length sequence. Here, the purification, crystallization and structural data of the full 102-amino-acid protein are presented. These data revealed that the presence of the C-terminus changes the crystal packing of E. coli Hfq. The crystal structure is discussed in the context of the recently published solution structure of Hfq from E. coli.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087635PMC
http://dx.doi.org/10.1107/S174430911100786XDOI Listing

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