AI Article Synopsis
- The study examines how different divalent metal ions (Ca²⁺, Mg²⁺, Co²⁺, Ni²⁺, Cu²⁺, Zn²⁺) affect DNA condensation and its stability in thermal conditions when mixed with DOPC liposomes.
- Ca²⁺, Mg²⁺, and Zn²⁺ promote DNA condensation, while Cu²⁺ destabilizes the DNA structure.
- Co²⁺ and Ni²⁺ show mixed results, sometimes stabilizing DNA but also contributing to its denaturation in certain conditions.
Article Abstract
We studied the effect of divalent alkaline earth metal cations Ca²⁺, Mg²⁺ and transition metals Co²⁺, Ni²⁺, Cu²⁺ and Zn²⁺ on DNA condensation and its protection against thermal denaturation in presence of dioleoylphosphatidylcholine liposomes (DOPC). Experimental results have shown that Ca²⁺ and Mg²⁺ as well as Zn²⁺ mediate DNA condensation. Cu²⁺ causes DNA double helix destabilization, and does not mediate binding between DNA and DOPC liposomes. Co²⁺ and Ni²⁺ can interact with DNA on both ways mentioned above. Static light scattering was use to follow the size of aggregates in DNA condensation process. Phospholipid bilayer and divalent cations protect condensed DNA against thermal destabilization. The highest stabilization effect was found in aggregates with Ca²⁺ and Zn²⁺, whereas in presence of either Co²⁺ or Ni²⁺ some volume fraction of DNA is denatured.
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| http://dx.doi.org/10.1016/j.colsurfb.2011.04.001 | DOI Listing |
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