Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase.

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Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, Japan.

Published: June 2011

AI Article Synopsis

  • This study showcases the use of bioluminescence to detect non-engineered RNAs in living cells.
  • Two types of probes were employed: the peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe.
  • The combination of these probes allowed for the direct detection of RNA that was introduced into the cells.

Article Abstract

In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells.

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Source
http://dx.doi.org/10.1039/c1an15130dDOI Listing

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