AI Article Synopsis
- Invitrogen's Gateway technology is a cloning method that simplifies the transfer of many open reading frames (ORFs) into plasmid vectors, making it effective for high-throughput purposes.
- MYXV, a poxvirus that infects only European rabbits and encodes 171 ORFs, uses this technology to create an ORF library, providing insights into its pathogenesis.
- MYXV shows potential for cancer treatment by targeting human cancer cell lines, making it a valuable model for researching poxvirus biology and developing oncolytic therapies.
Article Abstract
Invitrogen's Gateway technology is a recombination-based cloning method that allows for rapid transfer of numerous open reading frames (ORFs) into multiple plasmid vectors, making it useful for diverse high-throughput applications. Gateway technology has been utilized to create an ORF library for Myxoma virus (MYXV), a member of the Poxviridae family of DNA viruses. MYXV is the prototype virus for the genus Leporipoxvirus, and is pathogenic only in European rabbits. MYXV replicates exclusively in the host cell cytoplasm, and its genome encodes 171 ORFs. A number of these ORFs encode proteins that interfere with or modulate host defense mechanisms, particularly the inflammatory responses. Furthermore, MYXV is able to productively infect a variety of human cancer cell lines and is being developed as an oncolytic virus for treating human cancers. MYXV is therefore an excellent model for studying poxvirus biology, pathogenesis, and host tropism, and a good candidate for ORFeome development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104670 | PMC |
| http://dx.doi.org/10.1002/9780471729259.mc14a02s21 | DOI Listing |
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- The study focused on evaluating the effectiveness of dried blood spots (DBS) for serological surveys of these viruses in European rabbits by comparing DBS to traditional serum samples.
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