AI Article Synopsis
- The multiple target loci assembly sequencing (mTAS) method allows researchers to analyze multiple genomic loci in one DNA sequencing read through uniform amplification into a single fragment.
- Using polymerase cycling assembly (PCA), mTAS successfully gathered multiloci sequence data from a single sequencing run, applied to 29 sets of human genomic loci containing various single-nucleotide polymorphisms (SNPs).
- The mTAS approach has the potential to lower the costs associated with Sanger sequencing for genetic analysis.
Article Abstract
Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.
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| http://dx.doi.org/10.1016/j.ab.2011.04.012 | DOI Listing |
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