AI Article Synopsis
- Gao-Cha, a traditional Chinese tea from Acer ginnala, was analyzed for its medicinal properties and showed strong radical scavenging activity.
- The methanolic extract's IC(50) against the DPPH radical was determined to be 52.7 μg/mL, with three isolated radical scavengers demonstrating even greater potency.
- The study identified key bioactive compounds, including methyl 3,4,5-trihydroxybenzoate, quercetin-3-O-α-rhamnopyranoside, and 2,6-bis(3,4,5-trihydroxybenzoyl)-aceritol, with new insights into their galloyl structures.
Article Abstract
Gao-Cha is a traditional Chinese health tea made from Acer ginnala. We performed a components and radical scavenging activity analysis to identify any medicinal components in this tea. High performance thin layer chromatography (HPTLC)-1,1-Diphenyl-2-picryhydrazyl (HPTLC-DPPH) assay showed that the methanolic extract contained strong radical scavengers. Quantitative analysis revealed that the IC(50) of the extract against 1 mM DPPH was 52.7 ± 0.6 μg/mL. Bioactive-guided isolations led to procurement of 3 radical scavengers with IC(50)s of 17.5 ± 2.1, 29.3 ± 2.5, and 21.6 ± 1.7 μg/mL, respectively. Analysis of the high resolution-electric spray ionization-mass spectrometer and (1)H, (13)C, distortionless enhancement by polarization transfer at 135°, heteronuclear quantum coherence, correlating spectroscopy coupling, and heteronuclear multiple bond coherence (HMBC) data revealed that the compounds were methyl 3, 4, 5-trihydroxybenzoate (1), quercetin-3-O-α-rhamnopyranoside (2), and 2,6-bis (3,4,5-trihydroxybenzoyl)-aceritol (3). Bioactive combined components analysis revealed that, apart from compounds 1, 2, and 3, the tea possibly contained radical scavengers: ginnalin A (4) and B (5), 2″-O-Galloylquercitrin (6) and 3″-O-Galloyl-quercitrin (7). Compounds 2, 6, and 7 were isolated from Acer ginnala for the first time. The positions of the 2 galloyl moieties in compound 3 were unambiguously established by the HMBC spectrum for the first time.
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| http://dx.doi.org/10.1111/j.1750-3841.2010.01804.x | DOI Listing |
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