We observed how combined mechanical stimuli affect the proliferation and differentiation of pre-osteoblasts. For this research, a bioreactor system was developed that can simultaneously stimulate cells with cyclic strain and ultrasound, each of which is known to effectively stimulate bone tissue regeneration. MC3T3-E1 pre-osteoblasts were chosen for bone tissue engineering due to their osteoblast-like characteristics. 3-D scaffolds were fabricated with polycaprolactone and poly-L-lactic acid using the salt leaching method. The cells were stimulated by the bioreactor with cyclic strain and ultrasound. The bioreactor was set at a frequency of 1.0 Hz and 10 % strain for cyclic strain and 1.0 MHz and 30 mW/cm(2) for ultrasound. Three experimental groups (ultrasound, cyclic strain, and combined stimulation) and a control group were examined. Each group was stimulated for 20 min/day. Mechanical stimuli did not affect MC3T3-E1 cell proliferation significantly up to 10 days when measured with the cell counting kit-8. However, gene expression analysis of collagen type-I, osteocalcin, RUNX2, and osterix revealed that the combined mechanical stimulation accelerated the matrix maturation of MC3T3-E1 cells. These results indicate that the combined mechanical stimulation can enhance the differentiation of pre-osteoblasts more efficiently than simple stimuli, in spite of no effect on cell proliferation.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128915 | PMC |
http://dx.doi.org/10.3858/emm.2011.43.6.040 | DOI Listing |
Angew Chem Int Ed Engl
January 2025
University of Thessaly, Mechanical Engineering, Leoforos Athinon, Pedion Areos, 383 34, Volos, GREECE.
To accelerate the water dissociation in the Volmer step and alleviate the destruction of bubbles to the physical structure of catalysts during the alkaline hydrogen evolution, an integrated electrode of cobalt oxide and cobalt-molybdenum oxide grown on Ni foam, named CoO-Co2Mo3O8, is designed. This integrated electrode enhances the catalyst-substrate interaction confirmed by a micro-indentation tester, and thus hinders the destruction of the physical structure of catalysts caused by bubbles. Electrochemical testing shows the occurrence of a surface reconstruction of the integrated electrode, and CoO is transformed into Co(OH)2, denoted as Co(OH)2-Co2Mo3O8.
View Article and Find Full Text PDFSci Rep
January 2025
Institute of Mechanical Science, Vilnius Gediminas Technical University, Vilnius, 10105, Lithuania.
Digital transformation (DT) has become vital for companies trying to remain competitive in the recent ever-changing technological environment. DT is the integration of digital technologies into all disciplines of business from regular activities to strategic decision making. Risk management planning requires projects to assess possible risks that may negatively or positively affect a DT project.
View Article and Find Full Text PDFLife Sci Space Res (Amst)
February 2025
NASA Ames Research Center, Moffett Field, CA 94035, United States.
The BioSentinel CubeSat was deployed on the Artemis-I mission in November 2022 and has been continuously transmitting physical measurements of the space radiation environment since that time. Just before mission launch, we published computational model predictions of the galactic cosmic ray exposure expected inside BioSentinel for multiple locations and configurations. The predictions utilized models for the ambient galactic cosmic ray environment, radiation physics and transport, and BioSentinel geometry.
View Article and Find Full Text PDFJ Immunother Cancer
January 2025
Division of Tumor Immunology, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
Background: A number of immunotherapeutic approaches have been developed and are entering the clinic. Bispecific antibodies (BsAbs) are one of these modalities and induce robust efficacy by endogenous T cells in several hematological malignancies. However, most of the treated patients experience only a temporary benefit.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Plant Fibril Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangzhou 510006, China.
Untreated waste liquid mixtures often support large bacterial populations, posing challenges to effective purification due to high volume and limited filtration efficiency. This study aims to develop a multifunctional filtration membrane that combines both filtration and sterilization, enhancing overall purification efficiency. Using electrospinning technology, we fabricated a superhydrophilic, oil-repellent membrane by integrating the hydrophilic properties of chitosan, antibacterial N-halamine groups, and the mechanical strength of cellulose nanocrystals (CNC).
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!