The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131635 | PMC |
| http://dx.doi.org/10.1128/AEM.02289-10 | DOI Listing |
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