Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.
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Department of Pathology and Laboratory Medicine, Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Piwi proteins, such as Aubergine in Drosophila and Miwi and Mili in mice, form a major subclade of the Argonaute family, which comprise a distinct class of RNA-binding proteins (RBPs) able to bind small RNAs. Small RNAs can target complementary RNAs. Piwis are essential for the animal germline and bind Piwi-interacting RNAs (piRNAs) to form pi-RiboNucleoProteins (piRNPs).
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Methods Mol Biol
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Division of Neuropathology, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs.
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