AI Article Synopsis

  • Whole-cell patch-clamp analysis of primary osteoblasts and MG-63 osteoblast-like cells revealed a resting membrane potential of -60 mV and identified specific action potential characteristics including a duration of 60 ms and a threshold of -20 mV.
  • The study showed that inward currents occur at voltages below -60 mV, while depolarizing voltages above -30 mV produce both fast inward and delayed outward currents.
  • Expression of various Na(+) and K(+) channels was confirmed through RT-PCR, indicating that osteoblasts can generate action potentials due to the presence of these ion channels.

Article Abstract

Whole-cell patch-clamp analysis revealed a resting membrane potential of -60 mV in primary osteoblasts and in the MG-63 osteoblast-like cells. Depolarization-induced action potentials were characterized by duration of 60 ms, a minimal peak-to-peak distance of 180 ms, a threshold value of -20 mV and a repolarization between the spikes to -45 mV. Expressed channels were characterized by application of voltage pulses between -150 mV and 90 mV in 10 mV steps, from a holding potential of -40 mV. Voltages below -60 mV induced an inward current. Depolarizing voltages above -30 mV evoked two currents: (a) a fast activated and inactivated inward current at voltages between -30 and 30 mV, and (b) a delayed-activated outward current that was induced by voltages above -30 mV. Electrophysiological and pharmacological parameters indicated that hyperpolarization activated strongly rectifying K(+) (K(ir)) channels, whereas depolarization activated tetrodotoxin sensitive voltage gated Na(+) (Na(v)) channels as well as delayed, slowly activated, non-inactivating, and tetraethylammonium sensitive voltage gated K(+) (K(v)) channels. In addition, RT-PCR showed expression of Na(v)1.3, Na(v)1.4, Na(v)1.5, Na(v)1.6, Na(v)1.7, and K(ir)2.1, K(ir)2.3, and K(ir)2.4 as well as K(v)2.1. We conclude that osteoblasts express channels that allow firing of action potentials.

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http://dx.doi.org/10.1007/s10863-011-9354-7DOI Listing

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