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Establishment and characterization of canine rhabdomyosarcoma cell line CMS-C. | LitMetric

AI Article Synopsis

  • A new canine tumor cell line called CMS-C was created from a pleomorphic rhabdomyosarcoma found in a 14-year-old male dog.
  • The CMS-C cells showed similar characteristics to the original tumor, being positive for specific markers like vimentin and desmin, and negative for others.
  • This cell line is believed to originate from rhabdomyoblasts and will be valuable for future research on canine rhabdomyosarcoma.

Article Abstract

A novel canine tumor cell line designated as the CMS-C cell line was established from pleomorphic rhabdomyosarcoma (RMS) raised in the prostate gland of a 14-year-old intact male mixed-breed dog. CMS-C cells displayed the same immunohistochemical characteristics (positive for vimentin and desmin and negative for cytokeratin and smooth muscle actin) as the original tumor cells and express myoD1 and UCP3, known as striated muscle-specific molecules, as shown by RT-PCR assay. Therefore, the established CMS-C cell line appears to be of rhabdomyoblast cell origin. The CMS-C cell line established from pleomorphic RMS will be a useful tool for further studies about canine RMS.

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Source
http://dx.doi.org/10.1292/jvms.10-0436DOI Listing

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