The Marasmius oreades mushroom lectin (MOA) is well known for its exquisite binding specificity for blood group B antigens. In addition to its N-terminal carbohydrate-binding domain, MOA possesses a C-terminal domain with unknown function, which structurally resembles hydrolytic enzymes. Here we show that MOA indeed has catalytic activity. It is a calcium-dependent cysteine protease resembling papain-like cysteine proteases, with Cys215 being the catalytic nucleophile. The possible importance of MOA's proteolytic activity for mushroom defense against pathogens is discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2011.04.031 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fungal lectins show differential antiproliferative activity against cancer cell lines.
Int J Biol Macromol
December 2024
BioLab, Instituto Universitario de Bio-Orgánica "Antonio González", Universidad de La Laguna, La Laguna, Spain.
Glycosylation patterns represent an important signature of cancer cells that can be decoded by glycan-binding proteins, i.e., lectins.
View Article and Find Full Text PDFEvolution of a bipolar sexual compatibility system in .
Mycologia
January 2025
Department of Ecology, Environment and Plant Sciences, Stockholm University, Svante Arrhenius v. 20 A, Stockholm SE-114 18, Sweden.
Sexual compatibility in the Basidiomycota is governed by genetic identity at one or two loci, resulting in compatibility systems called bipolar and tetrapolar. The loci are known as and , encoding homeodomain transcription factors and pheromone precursors and receptors, respectively. Bipolarity is known to evolve either by linkage of the two loci or by loss of mating-type determination of either the or the locus.
View Article and Find Full Text PDFPhytochemical profile, bioactivity, and molecular docking studies of natural edible mushrooms grown in Tokat and Sivas provinces of Turkey.
J Food Sci
September 2024
Department of Chemistry, Faculty of Science, Ondokuz Mayis University, Samsun, Turkey.
Mushrooms have been essential to the human diet because they contain balanced chemical components and some biologically active substances. In this work, we investigated the phenolics, essential oils, metal contents, antioxidant, antibacterial, DNA protective, and enzyme inhibition activities for Clitocybe geotropa, Ramaria aurea, Rhizopogon luteolus (RL), Russula delica (RD), Verpa bohemica, and Marasmius oreades mushrooms. Results exhibited a higher content for citric and succinic acids in all tested kinds.
View Article and Find Full Text PDFModulation of adhesion to biotic model surfaces by fungal lectins and protease inhibitors.
Front Cell Infect Microbiol
May 2024
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Article Synopsis
- * The study tested different types of coordinated interactions between the bacterium, fungal lectins, and Caco-2 cells, finding that pre-incubation with lectins was most effective at reducing invasive bacterial cells.
- * These findings suggest that fungal lectins could be valuable in developing new treatments and preventive measures against such bacterial infections.
Multistep Biooxidation of 5-(Hydroxymethyl)furfural to 2,5-Furandicarboxylic Acid with HO by Unspecific Peroxygenases.
ChemSusChem
June 2024
Austrian Center of Industrial Biotechnology (ACIB GmbH), c/o Department of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.
Article Synopsis
- 5-(Hydroxymethyl)furfural (HMF) is a renewable biomass-derived chemical that can replace fossil fuel-derived materials, with 2,5-furandicarboxylic acid (FDCA) being a notable product resulting from HMF.
- The study showcases a biocatalytic method to synthesize FDCA from HMF through a one-pot, three-step oxidative process using unspecific peroxygenases (UPOs) and hydrogen peroxide under mild conditions.
- The research found that MorUPO successfully oxidized HMF with high selectivity and efficiency, achieving nearly complete conversion to FDCA and marking a significant step towards gram-scale biocatalytic synthesis of valuable chemicals.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!