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Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags.
Food Chem X
January 2025
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal structure. In parallel, three kinds of Raman dyes encoding Au@Ag, Au@Ag and Au@Ag SERS nanotags were synthesized for the detection of OTA, AFB1 and ZON.
View Article and Find Full Text PDFRatiometric fluorescent aptasensor based on DNA-gated FeO@Uio-66-NH and Exo I-assisted signal amplification.
Anal Chim Acta
February 2025
Food Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518045, PR China.
Background: Ochratoxin A (OTA) is toxic secondary metabolites produced by fungi and can pose a serious threat to food safety and human health. Due to the high stability and toxicity, OTA contamination in agricultural products is of great concern. Therefore, the development of a highly sensitive and reliable OTA detection method is crucial to ensure food safety.
View Article and Find Full Text PDFDetection of aflatoxin B level and revelation of its dynamic accumulation process using visible/near-infrared hyperspectral and microscopic imaging.
Int J Food Microbiol
January 2025
Beijing Key Laboratory of Optimization Design for Modern Agriculture Equipment, College of Engineering, China Agriculture University, Beijing 100083, China.
Understanding and controlling the dynamic process of aflatoxin B (AFB) accumulation by Aspergillus flavus (A. flavus) remains challenging. In this study, the A.
View Article and Find Full Text PDFDetermination of Puberulic Acid in Monascus-Fermented Red Yeast Rice by LC-MS/MS Combined with Precolumn Derivatization.
Toxins (Basel)
December 2024
NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Shanghai 201203, China.
Puberulic acid (PA) is a mycotoxin produced by a species of . It has received widespread attention as a significant contributor to the reported fatalities associated with red yeast rice dietary supplements. However, the detection of PA, especially at low concentration levels, poses a considerable challenge, with no detection methods reported thus far.
View Article and Find Full Text PDFTranscriptomic Analysis Reveals Molecular Mechanisms Underpinning Mycovirus-Mediated Hypervirulence in Infecting .
J Fungi (Basel)
January 2025
Hawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW 2753, Australia.
Mycoviral infection can either be asymptomatic or have marked effects on fungal hosts, influencing them either positively or negatively. To fully understand the effects of mycovirus infection on the fungal host, transcriptomic profiling of four isolates, including EABb 92/11-Dm that harbors mycoviruses, was performed 48 h following infection of via topical application or injection. Genes that participate in carbohydrate assimilation and transportation, and those essential for fungal survival and oxidative stress tolerance, calcium uptake, and iron uptake, were found to be overexpressed in the virus-infected isolate during the mid-infection stage.
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