AI Article Synopsis
- The study investigates the anticancer properties of bioactive components from fresh American ginseng berry extract (AGBE) on human colorectal cancer cells (SW480).
- HPLC analysis showed AGBE contains a specific profile of 8 ginsenosides, and the extract significantly inhibits cell growth in a time- and concentration-dependent manner.
- Results indicate that AGBE and the ginsenosides Rb3, Re, and Rg3 may hinder DNA replication and induce cell cycle arrest and apoptosis in SW480 cells.
Article Abstract
Purpose: The pharmacological activities, notably the anticancer properties, of bioactive constituents fromfresh American ginseng berry have not yet been well studied. In this study, we investigated the antiproliferative effects of fresh American ginseng berry extract (AGBE) and its representative triterpenoid glycosides using the human colorectal cancer cell line SW480.
Materials And Methods: Using high performance liquid chromatography (HPLC), the contents of 8 ginsenosides in AGBE were determined. The cell growth inhibitory effects of AGBE and three triterpenoid glycosides (ginsenosides Rb3, Re, and Rg3) were evaluated by proliferation assay and (3)H-thymidine incorporation assay. Cell cycle and apoptotic effects were analyzed by using flow cytometry after staining with propidium iodide and annexin V.
Results: HPLC analysis data showed that AGBE has a distinct ginsenoside profile. AGBE inhibited SW480 cell growth significantly in a time-dependent (24-96 hours) and concentration-dependent (0.1-1.0 mg/mL) manner. Ginsenosides Rb3, Re, and Rg3 also possess significant antiproliferative activities on SW480 cells. (3)H-thymidine incorporation assay indicated that AGBE and ginsenosides Rb3, Re, and Rg3 might inhibit the transferring and duplication of DNA in SW480 cells. Flow cytometric assay data suggested that AGBE arrested SW480 cells in S and G2/M phases, and significantly induced cell apoptosis.
Conclusion: AGBE and ginsenosides Rb3, Re, and Rg3 possessed significant antiproliferative effects and induced changes of morphological appearance on SW480 cells. The mechanisms of the antiproliferation of AGBE and tested ginsenosides involved could be cell cycle arrest and induction of apoptosis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072535 | PMC |
| http://dx.doi.org/10.4143/crt.2011.43.1.49 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mechanism of sodium nitroprusside regulating ginseng quality.
Sci Rep
January 2025
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, 150040, Hei-longjiang, China.
The roots of Panax ginseng C. A. Meyer (ginseng) are one of the traditional medicinal herbs in Asian countries and is known as the "king of all herbs".
View Article and Find Full Text PDFTranscriptome Profiling, Cloning, and Characterization of AnGlu04478, a Ginsenoside Hydrolyzing β-Glucosidase from Aspergillus niger NG1306.
Curr Microbiol
December 2024
College of Agriculture and Life Sciences, Kunming University, 2 Pu Xin Road, Kunming, 650214, Yunnan, China.
β-Glucosidase plays a pivotal role in transforming ginsenosides into specific minor ginsenosides. In this study, total ginsenosides from Panax notoginseng leaves were used as substrates to stimulate the growth of Aspergillus niger NG1306. Transcriptome analysis identified a β-glucosidase gene, Anglu04478 (1455 bp, 484 amino acids, 54.
View Article and Find Full Text PDFEffects of water stress on secondary metabolism of Panax ginseng fresh roots.
PLoS One
November 2024
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Article Synopsis
- Panax ginseng, a commonly used herbal medicine in Asia, relies on its roots and rhizomes, which contain ginsenosides, the main active compounds that enhance its adaptability to ecological stress.
- A study involved applying water spray to create a short-term water stress scenario for 5-year-old P. ginseng roots, revealing significant increases in oxidative stress indicators and enzyme activities linked to ginsenoside production.
- The water stress treatment resulted in notable boosts in various ginsenosides (e.g., Rg1 and Rb1) and a 40.1% increase in total saponins, highlighting the potential for water management to enhance the medicinal properties of P. ginseng.
Ginsenoside Rb3 Promotes Opa1-Mediated Regenerative Neurogenesis via Activating the Ido1 Pathway in Ischemic Stroke.
Phytother Res
November 2024
Institute of Neuroregeneration & Neurorehabilitation, Department of Pathophysiology, School of Basic Medicine, Qingdao University, Qingdao, China.
The activation of neural stem cells (NSCs) residing in the subventricular zone (SVZ) and dentate gyrus (DG) has been shown to promote the restoration of damaged brain tissues. Ginsenoside Rb3 (Rb3) is a bioactive substance known for its pharmacological properties in treating neurological disorders. This study investigated the effects of Rb3 on neural regeneration following ischaemic stroke (IS) and the underlying mechanisms involved.
View Article and Find Full Text PDFSystems Pharmacology to Explore the Potential Mechanism of Ginseng Against Heart Failure.
Rejuvenation Res
November 2024
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
The aim of this study is to elucidate the pharmacological mechanism underlying the effects of Ginseng Radix et Rhizoma (ginseng) in heart failure (HF), providing a theoretical foundation for its clinical application. The potential mechanism of ginseng in the context of HF was investigated using systems pharmacology that combined network pharmacology, Gene Expression Omnibus (GEO) analysis, molecular docking, and experimental verification. Network pharmacology was employed to identify drug-disease targets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!