AI Article Synopsis
- The study aimed to isolate and analyze a novel human gene, C17orf62-L, to understand its effects on cell viability and localization.
- The gene was successfully cloned using RT-PCR and showed structural features like a signal peptide and transmembrane domain, with expression observed in various cell lines.
- Functional tests indicated that overexpression of C17orf62-L leads to increased cell death, suggesting it plays a role in inducing apoptosis.
Article Abstract
Objective: To isolate the long coding sequence of human novel gene C17orf62 which is named as C17orf62-L by us, and analyze its effects on cell viability, subcellular localization, and expression profile in multiple cell lines.
Methods: RT-PCR (reverse transcription polymerase chain reaction ) was used to clone C17orf62-L which encoded 187 amino acids from human multi-tissue cDNA library. We used bioinformatics analysis to identify structural characteristics of C17orf62-L, and RT-PCR to detect its expression. By Laser Scanning Confocal Microscopy we identified its subcellular localization of C17orf62-L. Furthermore, flow cytometry experiment was used to validate whether overexpression of C17orf62-L could influence cell phenotypes and Western blot was used to study related mechanisms.
Results: C17orf62-L was cloned and constructed into the pcDNA-and pEGFP-expression plasmids. C17orf62-L had signal peptide and transmembrane domain.C17orf62-L was widely expressed in multiple cell lines and was validated partial co-localization with Golgi apparatus. Functional studies showed C17orf62-L could induce cell death accompanied with rising of cleaved PARP(poly ADP-ribose polymerase).
Conclusion: Human C17orf62 is a novel cell death inducing gene.
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[Study on the mechanism of C17orf62 induced cell death].
Beijing Da Xue Xue Bao Yi Xue Ban
April 2011
Department of Medical Immunology, Peking University School of Basic Medical Science, Peking University Center for Human Disease Genomics, Beijing, China.
Article Synopsis
- The study aimed to isolate and analyze a novel human gene, C17orf62-L, to understand its effects on cell viability and localization.
- The gene was successfully cloned using RT-PCR and showed structural features like a signal peptide and transmembrane domain, with expression observed in various cell lines.
- Functional tests indicated that overexpression of C17orf62-L leads to increased cell death, suggesting it plays a role in inducing apoptosis.
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