RNA interference (RNAi) has been established as an important tool for functional genomics studies and has great promise as a therapeutic intervention for human diseases. In mammalian cells, RNAi is conventionally induced by 19-27-bp RNA duplexes generated by hybridization of two complementary oligonucleotide strands (oligos). Here we describe a novel class of RNAi molecules composed of a single 25-28-nucleotide (nt) oligo. The oligo has a 16-nt mRNA targeting region, followed by an additional 8-10 nt to enable self-dimerization into a partially complementary duplex. Analysis of numerous diverse structures demonstrates that molecules composed of two short helices separated by a loop can efficiently enter and activate the RNA-induced silencing complex (RISC). This finding enables the design of highly effective single-oligo compounds for any mRNA target.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3096035 | PMC |
| http://dx.doi.org/10.1261/rna.2399411 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biofilm morphology and antibiotic susceptibility of methicillin-resistant (MRSA) on poly-D,L-lactide--poly(ethylene glycol) (PDLLA-PEG) coated titanium.
Biofilm
December 2024
Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Biodegradable polymeric coatings are being explored as a preventive strategy for orthopaedic device-related infection. In this study, titanium surfaces (Ti) were coated with poly-D,L-lactide (PDLLA, (P)), polyethylene-glycol poly-D,L-lactide PEGylated-PDLLA, (PP20)), or multi-layered PEGylated-PDLLA (M), with or without 1 % silver sulfadiazine. The aim was to evaluate their cytocompatibility, resistance to biofilm formation, and their potential to enhance the susceptibility of any biofilm formed to antibiotics.
View Article and Find Full Text PDFFunctional hydrogel empowering 3D printing titanium alloys.
Mater Today Bio
February 2025
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, 130041, Jilin, China.
Titanium alloys are widely used in the manufacture of orthopedic prosthesis given their excellent mechanical properties and biocompatibility. However, the primary drawbacks of traditional titanium alloy prosthesis are their much higher elastic modulus than cancellous bone and poor interfacial adhesion, which lead to poor osseointegration. 3D-printed porous titanium alloys can partly address these issues, but their bio-inertness still requires modifications to adapt to different physiological and pathological microenvironments.
View Article and Find Full Text PDFInclusion of gold ions in tiara-like nickel hexanuclear nanoclusters.
Nanoscale
January 2025
Research Institute for Science and Technology, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Tiara-like metal nanoclusters (TNCs) composed of group 10 transition metals and thiolates can easily change their number of polymerization and include various molecules or metal ions as guests within their ring structures. Therefore, they are expected to be applied in sensing, storage, and catalyst materials based on their selective inclusion characteristics. However, there are very few reports regarding the principles of selective inclusion for guest molecules/ions in TNCs.
View Article and Find Full Text PDFExosomes and microRNAs as mediators of the exercise.
Eur J Med Res
January 2025
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
MicroRNAs (miRNAs), also known as microribonucleic acids, are small molecules found in specific tissues that are essential for maintaining proper control of genes and cellular processes. Environmental factors, such as physical exercise, can modulate miRNA expression and induce targeted changes in gene transcription. This article presents an overview of the present knowledge on the principal miRNAs influenced by physical activity in different tissues and bodily fluids.
View Article and Find Full Text PDFDeep eutectic system enhanced oat protein extraction.
J Food Sci
January 2025
Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada.
Oats are a rich source of plant-based proteins owing to their nutritional value, diverse functions, and high abundance. However, traditional methods for extracting oat proteins (OPs), such as alkali solution acid precipitation (ASAP), can cause environmental pollution and potentially protein denaturation. In this work, we studied the use of deep eutectic solvents (DESs) and deep eutectic system (DESys)-based methods for OP extraction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!